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We narrowed to 1,131 results for: Ncl

Showing: 981 - 1000 of 1131 results

  1. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227709-plasmid-map-sequence-id-450930

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  4. pENTR-DMD-Donor

    Plasmid
    #60605
    Purpose
    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Insert
    Hygromycin resistant gene
    Use
    Knock-in donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    60605-plasmid-map-sequence-id-97115

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  5. HR-TERT-SV40-GFP

    Plasmid
    #71397
    Purpose
    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Tags
    SV-40 GFP insertion in TERT promotor
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71397-plasmid-map-sequence-id-128453

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  6. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid
    #108546
    Purpose
    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    3xFLAG
    Expression
    Mutation
    Changed F79 to G
    Promoter
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108546-plasmid-map-sequence-id-210054

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  8. mEos3.2FP

    Plasmid
    #203757
    Purpose
    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    NRas c-term (NRAS Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203757-plasmid-map-sequence-id-403337

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  9. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid
    #139457
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139457-plasmid-map-sequence-id-308472

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  10. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid
    #139458
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139458-plasmid-map-sequence-id-308461

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  11. 139H2_HC

    Plasmid
    #206201
    Purpose
    For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.
    Depositor
    Joost Snijder
    Article
    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202
    Insert
    anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
    Use
    Tags
    -AAAHHHHHHHH
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    206201-plasmid-map-sequence-id-408741

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  12. pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)

    Plasmid
    #160641
    Purpose
    Module for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    ERLexABDGal4AD / PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160641-plasmid-map-sequence-id-321669

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  17. PC1575

    Plasmid
    #232088
    Purpose
    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.
    Depositor
    Pranam Chatterjee
    Article
    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi
    Insert
    uAb with cloning Esp3I restriction site
    Use
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232088-plasmid-map-sequence-id-464073

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  18. Aquamarine-LC3B G120A-TdLanYFP

    Plasmid
    #228561
    Purpose
    Encodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter
    Depositor
    Giulia Bertolin
    Article
    Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Use
    Tags
    Aquamarine and TdLanYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228561-plasmid-map-sequence-id-457344

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  19. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid
    #202331
    Purpose
    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.
    Depositor
    Didier Stainier
    Article
    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.
    Inserts
    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    Use
    Tags
    base 238 C -> A mutation causing a PTC at codo…
    Expression
    Worm
    Mutation
    Promoter
    This is a promoter sequence for C. elegans eft-3,…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202331-plasmid-map-sequence-id-396314

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Showing: 981 - 1000 of 1131 results