Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 1961 - 2000 of 18759 results

  1. pEF1a-nSpCas9-NG

    Plasmid
    #171692
    Purpose
    Expresses nSpCas9-NG in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9-NG
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…
    Promoter
    EF1a
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171692-plasmid-map-sequence-id-339138

    Enlarge

  2. A3A-PBE-NG

    Plasmid
    #138141
    Purpose
    Targeted C-to-T in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-nCas9-NG-UGI-NLS
    Expression
    Plant
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138141-plasmid-map-sequence-id-311740

    Enlarge

  4. pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed

    Plasmid
    #154242
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbAB
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154242-plasmid-map-sequence-id-305991

    Enlarge

  5. pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed

    Plasmid
    #154241
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154241-plasmid-map-sequence-id-306043

    Enlarge

  6. pVE2_4_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS(rev)

    Plasmid
    #154237
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS(reversed)+CP_pcbC
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154237-plasmid-map-sequence-id-305989

    Enlarge

  7. pLM2_31_p40S_QfDBD_VP16AD_cp_ura3_DsRed_0xQUAS

    Plasmid
    #154231
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_ura3 (no QUAS control)
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    154231-plasmid-map-sequence-id-306059

    Enlarge

  8. pVE2_9_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_0xQUAS

    Plasmid
    #154227
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_pcbAB (no QUAS control)
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154227-plasmid-map-sequence-id-306042

    Enlarge

  9. pLM2_32_p40S_QfDBD_VP16AD_cp_nirA_DsRed_0xQUAS

    Plasmid
    #154230
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_nirA (no QUAS control)
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154230-plasmid-map-sequence-id-306041

    Enlarge

  10. pVE2_10_p40S_QfDBD_VP16AD_cp_penDE_DsRed_0xQUAS

    Plasmid
    #154228
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_penDE (no QUAS control)
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154228-plasmid-map-sequence-id-305993

    Enlarge

  11. pLM2_33_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS

    Plasmid
    #154224
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_pcbC (no QUAS control)
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154224-plasmid-map-sequence-id-305992

    Enlarge

  13. BM00369

    Plasmid
    #155338
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Publication
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
    Use
    Synthetic Biology
    Expression
    Plant
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155338-plasmid-map-sequence-id-311146

    Enlarge

  14. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid
    #115490
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    115490-plasmid-map-sequence-id-238520

    Enlarge

  17. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid
    #121821
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9 (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121821-plasmid-map-sequence-id-237374

    Enlarge

  19. Sniper2L

    Plasmid
    #193856
    Purpose
    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    193856-plasmid-map-sequence-id-382455

    Enlarge

  20. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138566-plasmid-map-sequence-id-274798

    Enlarge

  21. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid
    #121833
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    x-dCas9(3.7)/Dnmt3a-3L (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121833-plasmid-map-sequence-id-237387

    Enlarge

  22. SpyCas9 PE2*

    Plasmid
    #169850
    Purpose
    Mammalian Expression, SpyCas9 prime editor, optimized NLS
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9 PE*
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169850-plasmid-map-sequence-id-332905

    Enlarge

  23. JAB3479

    Plasmid
    #190417
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Publication
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
    Expression
    Plant
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190417-plasmid-map-sequence-id-393617

    Enlarge

  24. JAB2602

    Plasmid
    #190389
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Publication
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
    Expression
    Plant
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190389-plasmid-map-sequence-id-393424

    Enlarge

  29. BE3-NLS-NLS-P2A-BLAST

    Plasmid
    #135348
    Purpose
    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-2XNLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135348-plasmid-map-sequence-id-267697

    Enlarge

  30. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

    Enlarge

  35. pAAV-EF1a-DIO-CoChR-Kv2.1-GP2A-NLS-eGFP

    Plasmid
    #253381
    Purpose
    Cre-dependent, soma-targeted CoChR with nuclear GFP expression
    Depositor
    Ofer Yizhar
    Insert
    st-CoChR-GP2A-NLS-eGFP
    Use
    AAV
    Expression
    Mammalian
    Available since
    May 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    253381-plasmid-map-sequence-id-510497

    Enlarge

Showing: 1961 - 2000 of 18759 results