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MSCV-Tox2-IRES-eGFP

Plasmid

#165428

Purpose

Expresses TOX2 in mammalian cells

Depositor

Patrick Hogan, Anjana Rao

Article

Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

Insert

TOX2 (Tox2 Mouse)

Use

Tags

3xflag 5' of Tox2

Expression

Mammalian

Mutation

Promoter

5' LTR

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

Plasmid

#187954

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-hHDAC4-tagBFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-extGluc-NGFR

Plasmid

#104832

Purpose

extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

extGLuc
NGFR

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) WT-HA

Plasmid

#199660

Purpose

Transient expression of LYCHOS (GPR155) WT

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-P33-shR

Plasmid

#208359

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations (shRNA resistance), 33 aa p…

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Use

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad2

Plasmid

#89827

Purpose

Knockdown human Smad2 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD2 shRNA (SMAD2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v1

Plasmid

#134275

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

524 amino acids, isoform 1

Promoter

CMV

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lamp1 FLAG

Plasmid

#199750

Purpose

Lentiviral construct to stably express Lamp1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Lamp1 (Lamp1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc Metap2

Plasmid

#199685

Purpose

Transient expression of Metap2

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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His-MBP-IST1 N-terminal domain

Plasmid

#184805

Purpose

Bacterial expression of IST1 N-terminal domain

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

IST1 (IST1 Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

aa 1-187 only

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-734pUC19-tNGFR-P2A-PIK3R1

Plasmid

#186107

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

PIK3R1 (PIK3R1 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-713pUC19-tNGFR-P2A-CD45

Plasmid

#186109

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD45 (PTPRC Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-720pUC19-tNGFR-P2A-ZAP70

Plasmid

#186120

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

ZAP70 (ZAP70 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-735pUC19-tNGFR-P2A-PIK3CD

Plasmid

#186087

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

PIK3CD (PIK3CD Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-727pUC19-tNGFR-P2A-WASP

Plasmid

#186089

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WASP (WAS Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIG-714pUC19-tNGFR-P2A-SAP

Plasmid

#186091

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

SAP (SH2D1A Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-459 tNGFR-P2A-STAT6

Plasmid

#186070

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT6 (STAT6 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-458 tNGFR-P2A-STAT2

Plasmid

#186076

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT2 (STAT2 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-CHMP1B, S2C

Plasmid

#184803

Purpose

Bacterial expression of CHMP1B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

CHMP1B (CHMP1B Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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