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M15 pCS-twhh-nBeta gal

Plasmid

#17152

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Use

Tags

nuclear beta-gal

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMSCV-GFP

Plasmid

#86537

Purpose

Retrovirus expressing GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin I (human)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP-150TAG

Plasmid

#174076

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Use

Tags

His-6

Expression

Bacterial

Mutation

N150TAG

Promoter

T7

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hSURF4-FLAG

Plasmid

#159139

Purpose

Hu SURF4 -flag mammalian expression Gateway vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

surfeit 4 (SURF4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV (cytomegalovirus) enhancer and the MSV (mouse…

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLambda

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-RIG-I-Myc

Plasmid

#215872

Purpose

RIG-I-Myc expression vector for overexpression

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

Insert

RIG-I (RIGI Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP3A4

Plasmid

#67104

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP3A4 (CYP3A4 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJK2020

Plasmid

#171932

Purpose

express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

Depositor

Judith Kimble

Article

Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

Insert

Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR

Use

Tags

superfolder GFP, mCherry

Expression

Worm

Mutation

Promoter

-

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CYP2D6

Plasmid

#67090

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2D6 (CYP2D6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PT7

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pWUR703

Plasmid

#53082

Purpose

inducible TtAgoDM (D478A,D546A) expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Use

Tags

Strep-II tag

Expression

Bacterial

Mutation

D478A D546A

Promoter

T7

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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puc19-pCas13a

Plasmid

#118963

Purpose

Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

Depositor

Magdy Mahfouz

Article

Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

Insert

Cas13a

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pZR060_CROPseq-sgCtrl-CRISPRi

Plasmid

#180269

Purpose

Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pZR069_CROPseq-sgPTPRC-CRISPRi

Plasmid

#180271

Purpose

Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

PTPRC (CD45) sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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