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pC029 - Lw2Cas13a from Leptotrichia wadei F0279

Plasmid

#91919

Purpose

Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Lw2Cas13a and guide expression cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24-C840W

Plasmid

#28136

Depositor

Michelle Barton

Article

Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V and C840W

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1-dUBA

Plasmid

#74205

Purpose

Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed aa 877 to encode for premature stop codon

Promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pLVX puro TRIM21 C54Y - GFP

Plasmid

#116942

Purpose

Expression of TRIM21 C54Y -GFP fusion

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 C54Y-GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

C54Y

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-HA

Plasmid

#128102

Purpose

Express HA tagged Nxph4

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GFP-TAG

Plasmid

#158131

Purpose

Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Introduced a stop codon in place of the tyrosine …

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Skp2 WT

Plasmid

#81117

Purpose

bacterial expression plasmid of Skp2

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1P shABCB7_2

Plasmid

#102969

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_2 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shABCB7_1

Plasmid

#102968

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_1 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-6A-HA

Plasmid

#128103

Purpose

Express HA tagged Nxph4-6A

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pC031 - RcrCas13a from Rhodobacter capsulatus R121

Plasmid

#91921

Purpose

Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcrCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

Plasmid

#91917

Purpose

Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LweCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

Plasmid

#25458

Depositor

Betty Eipper

Article

Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

Insert

PAM (Pam Rat)

Use

Tags

His6 and PAM signal

Expression

Mammalian

Mutation

Contains aa 498-820 (PALcc= PAL catalytic core) …

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only