We narrowed to 159,238 results for: addgene

1. 5J39 (TDRKH)

   Plasmid

   #79523

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD2 (TDRKH Human)
   

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   June 16, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. PIWIL2

   Plasmid

   #32042

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PIWIL2 (PIWIL2 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   PAZ domain only (amino acids 386-533)

   Promoter

   Available since

   Jan. 3, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. integrin beta6 Y762F, Y774F pBabe puro

   Plasmid

   #13598

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 Y762F Y774F (ITGB6 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y762F Y774F

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. PHF13 (3O7A)

   Plasmid

   #28155

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PHD finger protein 13 (PHF13 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   PHD-type zinc finger domain (residues 250-301.

   Promoter

   Available since

   April 4, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pREST.1.0-gDNA

   Plasmid

   #113774

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor REST

   Depositor

   Kevin White

   Insert

   REST (REST Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p14-9VN9-3A

   Plasmid

   #50932

   Purpose

   Expresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.

   Depositor

   Katharina Strub

   Insert

   SRP14 and SRP9 (SRP14, SRP9 Human)
   

   Use

   Tags

   VN tag and flag tag

   Expression

   Mammalian

   Mutation

   Amino acids 60, 61 and 64 of SRP9 changed to alan…

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUK21-NotI

   Plasmid

   #51660

   Purpose

   Bacterial cloning vector based on pUK21 (Kan)

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiCRISPRv2-ACTB-C4

   Plasmid

   #229849

   Purpose

   Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-U6-MYC-Guide-hSpCas9

   Plasmid

   #228574

   Purpose

   Expression of human MYC gRNA; CRISPR mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   MYC gRNA (MYC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB_EF1a_mNeon

    Plasmid

    #212653

    Purpose

    piggyBac vector to express mNeon

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    mNeon
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCW57.1-RFP_mmu-miR-292b-3p_sponge

    Plasmid

    #132623

    Purpose

    Expresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDK362

    Plasmid

    #134194

    Purpose

    Retroviral plasmid with SKAP insert

    Depositor

    Iain Cheeseman

    Insert

    SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
    

    Use

    Retroviral

    Tags

    LAP (GFP)

    Expression

    Mammalian

    Mutation

    aa 166-316 with G5 and Jens siRNA hardening

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBRCA2.1.0-gDNA

    Plasmid

    #132448

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLF6.1.0-gDNA

    Plasmid

    #132435

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    KLF6 (KLF6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZNF462.1.0-gDNA

    Plasmid

    #112476

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462

    Depositor

    Kevin White

    Insert

    ZNF462 (ZNF462 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTBPL1.1.0-gDNA

    Plasmid

    #112412

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1

    Depositor

    Kevin White

    Insert

    TBPL1 (TBPL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMAD3.1.0-gDNA

    Plasmid

    #112407

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3

    Depositor

    Kevin White

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. WNK2 (WNK2A-c003)

    Plasmid

    #110250

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    WNK2 (WNK2 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5JJW (USP15_SART3)

    Plasmid

    #101880

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP15_SART3 (USP15, SART3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. 2MJ8

    Plasmid

    #61956

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDYL2 (CDYL2 Human)
    

    Use

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits