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Plasmid#115344PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDCC6
Plasmid#59985PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassetteDepositorInsertsCas9
U6-2>sgRNA
UseCRISPRTags3xFLAGExpressionMutationPromoterU6-96Ab and hsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYZ033
Plasmid#98404PurposeEntry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoterS.pombe rrk1 and nmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NLS-HA (pKW919)
Plasmid#229047PurposeMammalian expression of CasRx-NLS-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NES-HA (pKW387)
Plasmid#229048PurposeMammalian expression of CasRx-NES-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNES-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-HA (pKW88)
Plasmid#229046PurposeMammalian expression of CasRx-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-mB2M
Plasmid#154091PurposeSelf-Cutting and Integrating CRISPR backbone targeting murine B2MDepositorInsertsgRNA and BAIT targeting murine B2M locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST_116_LVL2 cam
Plasmid#179332PurposeNT-CRISPR plasmid for a single gRNA.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_119_LVL2 cam
Plasmid#179333PurposeNT-CRISPR plasmid for integration of multiple gRNAs.DepositorInsertPtac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-hTRAC
Plasmid#154094PurposeSelf-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chainDepositorInsertsgRNA and BAIT targeting human TRAC locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_2_VA
Plasmid#186438PurposeEncodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas12a(RuvC)
Plasmid#186447PurposeEncodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)DepositorInsertdCas12a
UseCRISPRTagsExpressionMutationD917A, E1006APromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas4_1_Δ2_VA
Plasmid#186439PurposeEncodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-mut4_1_2_VA
Plasmid#186441PurposeEncodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)DepositorInsertCas4, Cas1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1_2_VB
Plasmid#186442PurposeEncodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1 and Cas2
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-4/1Δ2_VB
Plasmid#186443PurposeEncodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)DepositorInsertCas4/1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only