Addgene

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MSCV-Mycn-IRES-RFP

Plasmid

#35393

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Mycn (Mycn Mouse)
RFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS(IRSp53 pLxIS)

Plasmid

#221315

Purpose

Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS)

Plasmid

#221240

Purpose

Expression of STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p6647 MSCV-P C-Flag HA 2aE7

Plasmid

#35137

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV2a E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
p6649 MSCV-P C-FlagHA 38 E7-Kozak

Plasmid

#35143

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV38 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-hCD2-CD14

Plasmid

#133061

Purpose

Retroviral expression of mouse CD14

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD14 (Cd14 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/Foxa1

Plasmid

#105506

Purpose

MSCV-driven retroviral Foxa1 expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-Ace-8aa-mScarlet

Plasmid

#129702

Purpose

Expresses Ace-8aa-mScarlet in mammalian cells

Depositor

Yiyang Gong

Article

Beck et al Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.

Insert

Ace-8aa-mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Camk2

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-mScarlet-ACTB

Plasmid

#207754

Purpose

Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAIDFA-CMV-NmScarlet-mAID

Plasmid

#140616

Purpose

Expression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1 mutated F74A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p6608 MSCV-IP N-HAonly CHAF1B

Plasmid

#34901

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHAF1B (CHAF1B Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-mScarlet-ACTB

Plasmid

#207753

Purpose

Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCL20c-MSCV-IRES-CRE

Plasmid

#86794

Purpose

expression in mammalian cells

Depositor

Martine Roussel, Charles Sherr

Insert

MSCV-IRES-CRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-ASNS-OE-IRES-Thy1.1

Plasmid

#192947

Purpose

Asparagine synthetase overexpression in murine cells

Depositor

Ping-Chih Ho

Article

Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

Insert

Asparagine synthetase (Asns Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Human HOXA9-MSCV-short

Plasmid

#20978

Depositor

Corey Largman

Article

Vijapurkar et al Mol Cell Biol. 2004 May . 24(9):3827-37.

Insert

HOXA9 (HOXA9 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Missing amino acid #1. Missing 3'UTR. Coding…

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF10

Plasmid

#105990

Purpose

retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF10 (Taf10 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_W267N

Plasmid

#214311

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

W267N

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Axin-IRES-GFP

Plasmid

#14716

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

Axin (Axin1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCRISPaint-mScarlet-PuroR [M1G]

Plasmid

#171809

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-PuroR

Use

Gene tagging

Tags

Expression

Mutation

PuroR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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