We narrowed to 4,130 results for: pCMV mammalian

1. pCMV-FLAG-RNF8

   Plasmid

   #221695

   Purpose

   Mammalian expression of FLAG-tagged human RNF8

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   RNF8 (RNF8 Human)
   

   Expression

   Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_SEPT9_i1 S111F-msfGFP

   Plasmid

   #180340

   Purpose

   mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S111F

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_SEPT9_i1 R106W-msfGFP

   Plasmid

   #180339

   Purpose

   mammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 R106W

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_SEPT9_i1 NCmut1-msfGFP

   Plasmid

   #180329

   Purpose

   mammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 R289A R290A K291A K294A

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_SEPT9_i1 D10-25-msfGFP

   Plasmid

   #180334

   Purpose

   mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 D10-25

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_SEPT9_i1 DN7-msfGFP

   Plasmid

   #180335

   Purpose

   mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 DN7

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_SEPT9_i1 Gmut-msfGFP

   Plasmid

   #180331

   Purpose

   mammalian expression of human SEPT9_i1 Gmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 W520A, H530D

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_SEPT9_i1 NCmut2-msfGFP

   Plasmid

   #180330

   Purpose

   mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 I281D, M288D

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV 3xFLAG-ATG3 1-13 KR

   Plasmid

   #129299

   Purpose

   Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed first 13 Lysine residues to Arginine

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV 3xFLAG-ATG3 14-22 KR

    Plasmid

    #129300

    Purpose

    Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed last 9 Lysine residues to Arginine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_mGL_PP7

    Plasmid

    #232440

    Purpose

    expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    mGreenLantern
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_CreNLS_PP7

    Plasmid

    #232439

    Purpose

    expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cre–MYC-NLS
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_RBMX-eA3A-UdgX-HF-nCas9

    Plasmid

    #163548

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE2-P2A-BSD

    Plasmid

    #174819

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-BSD
    

    Tags

    P2A-BSD and SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_UdgX-EE-UdgX-nCas9-UdgX

    Plasmid

    #163566

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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