We narrowed to 1,724 results for: pGEX

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pGEX-6P-1-HsTNRC6Biso1_1361-1723-6His_L

Plasmid

#146877

Purpose

Bacterial Expression of HsTNRC6Biso1_1361-1723

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1361-1723 (TNRC6B Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-CS-DmCup_311-440-Y342AL347A_M

Plasmid

#146916

Purpose

Bacterial Expression of DmCup_311-440-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_311-440-Y342AL347A (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207-EBM1mut_K

Plasmid

#146757

Purpose

Bacterial Expression of HsSmg6_1-207-EBM1mut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207-EBM1mut (SMG6 Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207-EBM2mut_K

Plasmid

#146758

Purpose

Bacterial Expression of HsSmg6_1-207-EBM2mut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207-EBM2mut (SMG6 Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsUPF3b-iso2_145-470_K

Plasmid

#146759

Purpose

Bacterial Expression of HsUPF3biso2_145-470

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF3biso2_145-470 (UPF3B Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-3-GST-DmGW182_1-593_F

Plasmid

#146271

Purpose

Bacterial Expression of DmGW182_1-593

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGW182_1-593 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutation T1772A (L591Q) compared t…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-PDE-short-UBASH3A

Plasmid

#195403

Purpose

Bacterial expression of a putative PDE domain of human short-form UBASH3A with GST tag

Depositor

Patrick Concannon

Insert

UBASH3A (UBASH3A Human)

Tags

GST

Expression

Bacterial

Mutation

PDE domain of UBASH3A

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(T304I)

Plasmid

#177133

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K72A)

Plasmid

#177132

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-G2304D

Plasmid

#182845

Purpose

mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Tags

GST

Expression

Bacterial

Mutation

mutation G2304D (GGC/GAC)

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRX-C751-E3616S

Plasmid

#182844

Purpose

mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRX (w Fly)

Tags

GST

Expression

Bacterial

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 4-178

Plasmid

#108284

Purpose

Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 179-199

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-NQO1(Y128F)

Plasmid

#177141

Purpose

Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

NQO1 (NQO1 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-optimized E6AP F727D

Plasmid

#165101

Purpose

ecoli optimized expression of a GST fusion of Human E6AP F727D

Depositor

Arthur Haas

Article

Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

Insert

E6AP F727D (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-HiGlpG- S116A, H196A

Plasmid

#158539

Purpose

Bacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196A

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

S116A;H169A

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-L77A/L80A

Plasmid

#100273

Purpose

Expresses GST-Stx3-L77A/L80A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-L77A/L80A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-E78A/E83A

Plasmid

#100272

Purpose

Expresses GST-Stx3-E78A/E83A in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-E78A/E83A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-LE165/166AA

Plasmid

#100271

Purpose

Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

Depositor

Thomas Weimbs

Article

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-LE165/166AA (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

tac

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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