We narrowed to 1,518 results for: pet-28a

1. GAL4-Creb5 (1- 128)

   Plasmid

   #195031

   Purpose

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL4-Creb5 (1- 128) T59/T61 A

   Plasmid

   #195032

   Purpose

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T59/T61 A

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GAL4-Creb5 (1- 128) C18/C23 S

   Plasmid

   #195033

   Purpose

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C18/C23 S

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ppwd1.473.646.W601A

   Plasmid

   #137660

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Melissa Jurica, Tara Davis

   Article

   Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

   Insert

   ppwd1 (PPWD1 Human)
   

   Use

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   473-646-W601A

   Promoter

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCRW1

   Plasmid

   #122462

   Purpose

   Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells

   Depositor

   Richard C. Willson

   Article

   Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10

   Insert

   NPM-ALK fusion protein (ALK, NPM1 Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac promoter

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Reb_81 aka PAS622

   Plasmid

   #71227

   Purpose

   Reb_1 with RebB(T28A, D72G)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebB(T28A, D72G), RebA(G53A)

   Promoter

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. CRBN_ΔHBD

   Plasmid

   #232779

   Purpose

   Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

   Depositor

   Ivan Dikic

   Article

   Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

   Insert

   Cereblon (CRBN Human)
   

   Use

   Tags

   MBP-His6-TEV

   Expression

   Bacterial

   Mutation

   CRBN (residues 47 to 193 and 249 to 436) with GNG…

   Promoter

   T7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Human RNF144B (27-236)

   Plasmid

   #110753

   Purpose

   Bacterial Expression of RNF144B RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144B (RNF144B Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p-mCherry-N1-iChloC_CA

   Plasmid

   #98171

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iChloC_C128A gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, C128A, T159C, D156N

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ppil2.280.457.Y389H

    Plasmid

    #137658

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Melissa Jurica, Tara Davis

    Article

    Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

    Insert

    ppil2 (PPIL2 Human)
    

    Use

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    280-457-Y389H

    Promoter

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ppil2.280.457.Y389W

    Plasmid

    #137657

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Melissa Jurica, Tara Davis

    Article

    Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

    Insert

    ppil2 (PPIL2 Human)
    

    Use

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    280-457-Y389W

    Promoter

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p-mCherry-C1-iC++_CA

    Plasmid

    #98168

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iC++_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p-mCherry-C1-Phobos_CA

    Plasmid

    #98169

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p-mCherry-C1-Aurora_CA

    Plasmid

    #98170

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JPUB_006818

    Plasmid

    #87924

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste3608
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JPUB_006812

    Plasmid

    #87925

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste2803
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ppj184 (JBEI-13431)

    Plasmid

    #89784

    Purpose

    for expression of N-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CpX-1

    Plasmid

    #218676

    Purpose

    Expresses complexin-1 in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    Rat complexin-1 (Cplx1 Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGJK_His-SUMO-LbuCas13a

    Plasmid

    #172488

    Purpose

    Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    bdSUMO-LbuCas13a (codon-optimized)
    

    Use

    Tags

    His6 and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 FLAG 17xS/T->A, catalytic active lipin 1

    Plasmid

    #32007

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

    Promoter

    CMV, SP6

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only