Addgene

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KBB285

Plasmid

#185076

Purpose

RFA3-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA3

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB284

Plasmid

#185075

Purpose

RFA2-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA2

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pVHed02

Plasmid

#82352

Purpose

Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

mKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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ECYFP

Plasmid

#111204

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2001 Feb 9;276(6):3805-10.

Insert

ECFP-Xpress tag-GFP10C fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDHt-KS

Plasmid

#63616

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Tags

Expression

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pdelta105-ABCb10-GFP

Plasmid

#19034

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

ABCb10 lacking the mitochondrial targetting sequence

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pro::EX1-2:GFP

Plasmid

#218565

Purpose

Transcriptional reporter for ARF7 promoter

Depositor

Anthony Bishopp

Insert

AT5G20730 (NPH4 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pro::EX1:GFP

Plasmid

#218564

Purpose

Transcriptional reporter for ARF7 promoter

Depositor

Anthony Bishopp

Insert

AT5G20730 (NPH4 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pro::GFP

Plasmid

#218563

Purpose

Transcriptional reporter for ARF7 promoter

Depositor

Anthony Bishopp

Insert

AT5G20730 (NPH4 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1177

Plasmid

#217961

Purpose

Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3 (HIST2H3PS2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV128-Rluc-CTE-CCC_O

Plasmid

#147107

Purpose

Mammalian Expression of Rluc-CTE-CCC

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

Rluc-CTE-CCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV128-Rluc-CTE-GAG_O

Plasmid

#147108

Purpose

Mammalian Expression of Rluc-CTE-GAG

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

Rluc-CTE-GAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HIV-Rev-M10_O

Plasmid

#147112

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev_O

Plasmid

#147113

Purpose

Mammalian Expression of HIV-Rev

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev-M10_O

Plasmid

#147114

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB329

Plasmid

#185077

Purpose

NUP82 expression in yeast

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

NUP82

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Use

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only