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Showing: 981 - 1000 of 4920 results

  2. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

    Plasmid
    #222650
    Purpose
    For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term
    Depositor
    Silvia Monticelli
    Article
    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024
    Insert
    Zc3h12a (Zc3h12a Mouse)
    Use
    In vitro transcription
    Tags
    FLAG and HA
    Expression
    Mutation
    mutated aspartic acid 141 to asparagine to abroga…
    Promoter
    T7
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    222650-plasmid-map-sequence-id-448254

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  3. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  10. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  11. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  12. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  13. pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)

    Plasmid
    #159222
    Purpose
    Can be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpO
    Depositor
    Rui Benedito
    Article
    Luo et al Nature. 2021 Jan;589(7842):437-441. doi:
    Insert
    Apln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Apln
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159222-plasmid-map-sequence-id-315768

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  19. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210743
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 3
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210743-plasmid-map-sequence-id-420083

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  20. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid
    #210744
    Purpose
    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp Cas9
    Merry sgRNA GFP Target 4
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210744-plasmid-map-sequence-id-420033

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Showing: 981 - 1000 of 4920 results