We narrowed to 7,351 results for: ski

1. PB-EEA-g1-PGK-Puro

   Plasmid

   #102908

   Purpose

   PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G07

   Plasmid

   #67141

   Purpose

   Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   His-MYC and mCherry

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFRB-myc

   Plasmid

   #20226

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   1 FRB copy has a T2098L point mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

4. MAGE-A3Pro pGL4basic

   Plasmid

   #78138

   Purpose

   luciferase reporter plasmid

   Depositor

   Heidi Schwarzenbach

   Article

   Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

   Insert

   MAGE-A3 (MAGEA3 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   contains -418/+12 of promoter

   Promoter

   MAGE-A3

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 RUVBL1

   Plasmid

   #67091

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 IL2

   Plasmid

   #67053

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL2 (IL2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCI-MEG3b

   Plasmid

   #59421

   Purpose

   Expresses lncRNA MEG3 isoform b in mammalian cells.

   Depositor

   Anne Klibanski

   Article

   Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

   Insert

   MEG3b (MEG3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-MEG3a

   Plasmid

   #59420

   Purpose

   Expresses lncRNA MEG3 isoform a in mammalian cells.

   Depositor

   Anne Klibanski

   Article

   Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

   Insert

   MEG3a (MEG3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Pr-deGFP-MGapt

   Plasmid

   #67734

   Purpose

   OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

   Depositor

   Richard Murray

   Article

   Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

   Insert

   UTR1-deGFP-MGapt-T500
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G08

    Plasmid

    #67142

    Purpose

    Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His-MYC and mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pecALPS gag-gfp Blasti

    Plasmid

    #115805

    Purpose

    Express gag-gfp fusion driven by EIF1a promoter

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EIF1a

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pAPM D4 BsmBI Myd88 stuffer

    Plasmid

    #128505

    Purpose

    pAPM knockdown vector with Myd88 stuffer sequence

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    Puromycin Resistance
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. XE101 alpha transducin CS2+

    Plasmid

    #16681

    Depositor

    Randall Moon

    Article

    Slusarski et al Nature. 1997 Nov 27;390(6658):410-3. doi

    Insert

    alpha-transducin (GNAT1 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCI-MEG3c

    Plasmid

    #59422

    Purpose

    Expresses lncRNA MEG3 isoform c in mammalian cells.

    Depositor

    Anne Klibanski

    Article

    Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

    Insert

    MEG3c (MEG3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ptcALPS gag-gfp Blasti

    Plasmid

    #115806

    Purpose

    Express gag-gfp fusion driven by TK promoter

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TK

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pFA6a-link-GFPγ-SpHis5

    Plasmid

    #60781

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPγ
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    T65C S72A V163A I167T L231H

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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