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EGFP-Rab8b in pCSDEST2

Plasmid

#140881

Purpose

Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8b (rab8b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a in pCSDEST2

Plasmid

#140880

Purpose

Expression of zebrafish GFP-tagged Rab8a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8a (rab8a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-KD

Plasmid

#140297

Purpose

Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-EB-Hyg-R4R3

Plasmid

#139520

Purpose

Provides episomal replication of the destination vector in primate cells

Depositor

Minoru Ko

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 LacZ

Plasmid

#123292

Purpose

Mammalian expression of control truncated LacZ

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

LacZ

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-FLP(D)

Plasmid

#119895

Purpose

FLP(D) with a hsp70 promoter

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Insert

FLP(D)

Use

Drosophila expression

Mutation

mutated form of FLP, which at position 5 contains…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design1

Plasmid

#100571

Purpose

FISSEQ-hgRNA-Design1 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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252 pTol2-MTDest

Plasmid

#73485

Purpose

promoterless Tol2 vector with 6xmyc tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

6xmyc/attR1/attR2

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL6-V5-His6

Plasmid

#67443

Purpose

Mammalian expression of hArl6 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL8B-V5-His6

Plasmid

#67446

Purpose

Mammalian expression of hArl8b with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-SARA-V5-His6

Plasmid

#67452

Purpose

Mammalian expression of hSara with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL3

Plasmid

#67418

Purpose

Expression of hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pETCON-HB20/design_70

Plasmid

#30254

Depositor

David Baker

Article

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

SC1918/H1 HA designed binding protein HB20

Expression

Yeast

Available Since

June 10, 2011

Availability

Academic Institutions and Nonprofits only
pDEST40-DJ1-E18N

Plasmid

#29407

Depositor

Mark Cookson

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Glu18Asn

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-d

Plasmid

#14087

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-d

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-d

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pW17

Plasmid

#158207

Purpose

2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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