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Showing: 10021 - 10040 of 65831 results

  3. Epac-S-H30

    Plasmid
    #170337
    Purpose
    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170337-plasmid-map-sequence-id-344639

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  4. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

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  5. pEF NS34a-msGFP-'S-mito

    Plasmid
    #158765
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158765-plasmid-map-sequence-id-313128

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  10. pEV_S

    Plasmid
    #100266
    Purpose
    pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli
    Depositor
    Timothy Lu
    Publication
    Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17.
    Inserts
    entD (entD Escherichia coli)
    entC
    entE
    entB
    entA
    vibH
    vibF
    cos
    Use
    Synthetic Biology; Expression, pbr322
    Expression
    Bacterial
    Available since
    Nov. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100266-plasmid-map-sequence-id-193235

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  14. pET15b-Fab1 (S. aureus)

    Plasmid
    #61691
    Purpose
    Inducible expression of the FabI enzyme from S. aureus
    Depositor
    Michael Johnson
    Insert
    FabI
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    49061-plasmid-map-sequence-id-72183

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Showing: 10021 - 10040 of 65831 results