We narrowed to 17,818 results for: multi
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Plasmid#216733PurposeExpresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.DepositorArticleInsertCas9-D10A nickase and sgCTG
UseCRISPR and LentiviralExpressionMammalianMutationD10APromoterU6 and CAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GABBR1-DuET
Plasmid#213247PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AVPR2-DuET
Plasmid#213188PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM1383
Plasmid#207423PurposeExtrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)DepositorAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BSD-P2A-TagRFP-hSmad1-donor
Plasmid#203662PurposeDonor plasmid for CRISPR knock-inDepositorAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
ExpressionPlantPromoterATUBI10 and Medtr8g059790(KPI)Available SinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx30
Plasmid#188988PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)DepositorInsertPLD
TagsCRY2 and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)
Plasmid#192124PurposepHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
ExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A
Plasmid#191676Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30ADepositorUseLentiviralTags6x HIS and flagMutationD433NAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_TOMM20-STf
Plasmid#182002PurposeCMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell linesDepositorInsertTOMM20-STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-LN2
Plasmid#188235PurposePolycistronic human LIN28 and NANOG were cloned into the pMXs vectorDepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP2-T2A-HO1
Plasmid#179485PurposeExpresses miRFP2 and HO1in worm cellsDepositorInsertwmIRFP2-T2A-HO1
ExpressionMammalianMutationNOPromotertag-168 promoterAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
Tags6xHis tag and HRV3C cleavage siteExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript II SK-SNAP donor for Hist1h3g
Plasmid#186932PurposeGenomic targeting of SNAP tag at Hist1h3g C-terminalDepositorAvailable SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-com-BoxB-vector
Plasmid#132556PurposeExpresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.DepositorInsertsdCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
Lambda N22-KRAB
sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
ExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
Plasmid#182346PurposePacI-flanked CAG-LSL expression cassette with a FNF for positive selectionDepositorInserttdTomato
UseMouse Targeting; Flp / frtExpressionMammalianAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only