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MS4A1 RFP (805)

Plasmid

#62009

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

MS4A1

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pTW094

Plasmid

#64757

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-6xHIS epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMT005

Plasmid

#64760

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an HA-6xHIS epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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C12-bio-His

Plasmid

#52178

Purpose

Expresses full-length Spexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

C12 (SPX Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR-CENPBi2

Plasmid

#59619

Purpose

Expression of shRNA against human CENPB

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

CENPB (CENPB Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM29i2

Plasmid

#59607

Purpose

Expression of shRNA against human ADAM29

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM29 (ADAM29 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Talin-ABM-10

Plasmid

#55510

Purpose

Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Talin ABM

Tags

mTFP1

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSC-hXRCC1-K176R-IntE2

Plasmid

#52277

Purpose

Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K176R, SUMO acceptor site mutant

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-hXRCC1-K176R-PreE2

Plasmid

#52279

Purpose

Bacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

Ubc9-GST

Expression

Bacterial

Mutation

K176R, SUMO acceptor site mutant

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-mTDG-K341R-IntE2

Plasmid

#52269

Purpose

Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Tags

GyrA-intein-Ubc9-GST

Expression

Bacterial

Mutation

K341R, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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CS2-mCHY-dASUN

Plasmid

#47042

Purpose

Fluorescent reported for dASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (asun Fly)

Tags

mCHY

Expression

Mammalian

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
CS2-HA-dASUN

Plasmid

#47041

Purpose

Expression of tagged-dASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (asun Fly)

Tags

HA

Expression

Mammalian

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Frmd4b

Plasmid

#45867

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Frmd4b in situ probe (Frmd4b Mouse)

Use

In situ

Mutation

contains bp# 3933-4535 of NM_145148.2

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-PTK9L

Plasmid

#31587

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PTK9L protein tyrosine kinase 9-like (A6-related protein) (TWF2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-308

Plasmid

#22708

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 308 (mir-308 primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1-GST

Plasmid

#41613

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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Abr-pLEF

Plasmid

#38157

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

ABR (ABR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00163

Plasmid

#34316

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2692 (gsb-n Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pTH443-SUP45-G180A

Plasmid

#29386

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

G180A mutant gene including genomic sequence 1432…

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only