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tagRFP-IMBB4-pUC

Plasmid

#170769

Purpose

test plasmid for 5RM assembly, encodes fluorescent red protein

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

tagRFP

Promoter

none

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-PPP2R5C-2

Plasmid

#179356

Purpose

shRNA suppression

Depositor

William Hahn

Article

Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

Insert

B56γ

Use

Lentiviral

Expression

Mammalian

Promoter

Age1

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-PPP2R3B-1

Plasmid

#179360

Purpose

shRNA suppression

Depositor

William Hahn

Article

Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

Insert

PR48

Use

Lentiviral

Expression

Mammalian

Promoter

Age1

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKM634

Plasmid

#134219

Purpose

Baculovirus expression of CENP-HIKM

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP HIKM

Expression

Insect

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pKM412

Plasmid

#134213

Purpose

Baculovirus expression of CENP-P and CENP-O

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENP-P + O

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEYD1A

Plasmid

#127759

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in divergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph18

Plasmid

#133029

Purpose

Expresses Xph18 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph18

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFast-dCry-V5His

Plasmid

#136240

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Insect

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph15

Plasmid

#133030

Purpose

Expresses Xph15 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph15

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTF101.4

Plasmid

#134773

Purpose

A binary plasmid containing nopaline synthase promoter to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEYT1A

Plasmid

#127760

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in tandem orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKD288

Plasmid

#125075

Purpose

Expression of SO_4638 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4638 (SO_4638 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAW207

Plasmid

#113247

Purpose

E. coli expression vector for IHFa K20A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K20A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K20A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHES944

Plasmid

#112642

Purpose

express SST2 from the GAL1 promoter

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

SST2

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC104650.1-1/1

Plasmid

#91541

Purpose

Protein expression and purification of human SH3 domain construct AC104650.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC104650.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC093799.1-1/1

Plasmid

#91495

Purpose

Protein expression and purification of human SH3 domain construct AC093799.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC093799.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-2/2

Plasmid

#91382

Purpose

Protein expression and purification of human SH3 domain construct OTOR-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-2/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-1/2

Plasmid

#91328

Purpose

Protein expression and purification of human SH3 domain construct OTOR-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-1/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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