We narrowed to 10,841 results for: esp
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Plasmid#90345PurposeSRF - Serum response factor gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterSRFAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
TagsEGFPExpressionBacterialPromoterPemrRAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.INBox-mCherry-INBox-eDHFR
Plasmid#176203PurposeDouble INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertINBox-mCherry-INBox-eDHFR
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-mEmerald-ER-5
Plasmid#170715PurposeExpresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.DepositorInsertAPV-DsRed2-mEmerald-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox-eDHFR
Plasmid#176202PurposeSingle INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertmCherry-INBox-eDHFR
ExpressionMammalianAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG.MCAK-mCherry-eDHFR
Plasmid#176204PurposeMCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization systemDepositorInsertMCAK-mCherry-eDHFR
ExpressionMammalianAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox(AAA)-eDHFR
Plasmid#176207PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization systemDepositorInsertmCherry-INBoxAAA-eDHFR
ExpressionMammalianAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC8
Plasmid#170757PurposeCo-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.DepositorTypeEmpty backboneTagssite 1- V5 tag and site 2- FLAG tagExpressionMammalianPromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-wtBlc
Plasmid#168256PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondinglyDepositorInsertLeucine Zipper + C-fragment of wtBlc
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-ChPylTMSK
Plasmid#163915PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165V
Plasmid#158965PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165V
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanR - F165R
Plasmid#158966PurposeWild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.DepositorInsertVanR - F165R
ExpressionYeastMutationWild type VanR from Caulobacter crescentus with m…PromoterTEF1Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXW101Plux2-Pecf11-gfp
Plasmid#160823PurposeTwo-layered AHL-responsive circuit carrying an ECF11-based amplifierDepositorInsertPJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterPJ23101, Plux2, Pecf11Available SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSO9
Plasmid#106366PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.DepositorInsertttTi4348_ SEC _pvit-6_OspF_3'unc54
ExpressionWormAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterPGKAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby
Plasmid#216225PurposeTDP-43 with a C-terminal mRuby tag for expression in mammalian cells.DepositorAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE56
Plasmid#247034PurposeGlucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.DepositorInsertFirefly luciferase
UseLentiviralPromoterNR3C1 (GR)Available SinceNov. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable SinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only