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TPC1(ΔN69)-mCherry

Plasmid

#135184

Purpose

TPC1 with truncated N-terminus, tagged with mCherry on C terminus.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 1-69

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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mouse a2b full length

Plasmid

#217812

Purpose

Full length mouse integrin a2b expression

Depositor

Timothy Springer

Article

Hao et al MAbs. 2024 Jan-Dec;16(1):2365891. doi: 1

Insert

ITGA2B (Itga2b Mouse)

Tags

P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

Plasmid

#167943

Purpose

SFTPC reporter targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SFTPC (SFTPC Human)

Use

CRISPR; Donor template

Tags

Venus

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

EF1a

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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TPC2(ΔN49)-mCherry

Plasmid

#135185

Purpose

TPC2 with truncated N-terminus, tagged with mCherry on C terminus.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino acids 1-49

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 prodomain-VC155

Plasmid

#49261

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Tags

HA and split Venus C

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBIFC-flag-hCaspase 2 prodomain-VN173

Plasmid

#49262

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pGV3319

Plasmid

#170277

Purpose

Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

Bst-LF D720A

Tags

Hexahistidine

Expression

Bacterial

Mutation

D720A

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pY036_ATP1A1_G3_Array

Plasmid

#86619

Purpose

Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA

Use

CRISPR; Co-selection via nhej using ouabain

Tags

3xHA

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 CARD-VC155

Plasmid

#49259

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

Caspase-2 CARD (CASP2 Human)

Tags

HA and split Venus C

Expression

Mammalian

Mutation

deleted aa 123-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 FL (C303S)-VN173

Plasmid

#49258

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pXPR_003 sgSMARCA4 guide 2

Plasmid

#193605

Purpose

SMARCA4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSMARCA4 guide 2 (SMARCA4 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-TEV-PWWP_DNMT3A

Plasmid

#186970

Purpose

Bacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

DNMT3A PWWP domain (DNMT3A Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Mutation

PWWP domain (residues 278–427)

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBiFC-flag-hCaspase 2 CARD-VN173

Plasmid

#49260

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 CARD (CASP2 Human)

Tags

Flag and split Venus N

Expression

Mammalian

Mutation

delete aa 123-435

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
APEX2-6xHp

Plasmid

#214411

Purpose

Lipid droplet localization of APEX2-6xHp for electron microscopy

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (APEX2 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

Plasmid

#156162

Purpose

Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 383-548

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV_PP1-mCitrine

Plasmid

#172472

Purpose

Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

Depositor

Sean R Collins

Article

Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

Insert

prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)

Use

Lentiviral

Tags

mCitrine and prolactin signal sequence (cleaved p…

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hLMX1A-T2A-copGFP

Plasmid

#170444

Purpose

Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human LMX1A

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_THRA_Hormone-recep

Plasmid

#109810

Purpose

Protein expression and purification of THRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

THRA_Hormone-recep (THRA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTD80

Plasmid

#172901

Purpose

Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCINeo-mCHMP5

Plasmid

#11771

Depositor

Sankar Ghosh

Article

Shim et al J Cell Biol. 2006 Mar 27. 172(7):1045-56

Insert

chromatin modifying protein 5 (Chmp5 Mouse)

Expression

Mammalian

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only