We narrowed to 12,355 results for: pcdna

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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_10_0460_E265A

Plasmid

#171641

Purpose

Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

Insert

LmjF.10.0460 E265A

Tags

Myc-His

Expression

Mammalian

Mutation

E265A

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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PA-RL-mCherry

Plasmid

#78861

Purpose

DULIP negative control (bait) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMM585-Tier1-PhCMV-Igk

Plasmid

#169486

Purpose

Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Igk secretion signal (IGK Human)

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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hP14Gal4

Plasmid

#84364

Purpose

Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

Gal4 and HA tag

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CMV/TO

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFOX6-Tier1-PhCMV-TdTomato

Plasmid

#169515

Purpose

Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven dimeric red fluorescent protein TdTomato

Expression

Mammalian

Promoter

PhCMV

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
nGR2175-gs10-NLS-VPR

Plasmid

#188264

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nGR2175

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Cav1-mRed

Plasmid

#12681

Depositor

Richard Pagano

Article

Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

Insert

canine caveolin-1 (CAV1 Canis familiaris)

Tags

mRed

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
OSCA1.2-PP-GFP

Plasmid

#135390

Purpose

Expresses OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Tags

EGFP tag connected to the gene via a precision pr…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Cenp-B DBD INCENP mCherry

Plasmid

#45233

Purpose

Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B-DBD INCENP-Δcen mCherry

Tags

DNA binding domain of CENP-B and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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mc2 blast EF1a

Plasmid

#206223

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_HEN1

Plasmid

#167708

Purpose

Expresses HEN1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

HEN1 (NHLH1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-Amida

Plasmid

#15362

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pOVC1_EcSSPB-nano_X

Plasmid

#131212

Purpose

Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-nano

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
Ilmol

Plasmid

#120310

Purpose

A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

Depositor

Bradley Baker

Article

Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

Insert

Ilmol

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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L1-A426D

Plasmid

#15485

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-A426D (L1CAM Human)

Expression

Bacterial

Mutation

Alanine 426 changed to Aspartic Acid

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
HA PRAS40 S183A

Plasmid

#15674

Depositor

John Lawrence

Article

Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

Insert

PRAS40 S183A (AKT1S1 Human)

Tags

HA

Expression

Mammalian

Mutation

S183A

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only