We narrowed to 18,247 results for: multi
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Plasmid#213379PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSSTR4 (SSTR4 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR1D-DuET
Plasmid#213318PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVExpressionMammalianPromoterU6Available sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEditA-RY
Plasmid#207533PurposePlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertxylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0015
Plasmid#205584PurposeNpuDnaE(C)_HYG(90-341)_RUBYDepositorInsertNpuDnaE(C)
ExpressionPlantPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Puro-T2A-mCitrine-hSmad2-donor
Plasmid#203663PurposeDonor plasmid for CRISPR knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSmad2
TagsmCitrineExpressionMammalianAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-DENV_NS3-GS-NS2B
Plasmid#201940PurposeExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-181
Plasmid#195429PurposeTransient, constitutive expression of synZiFTR: ZF6-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF6-4 (ZF6) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-mNeongreen_CydAB-mCherry
Plasmid#182391PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
cydAB-mCherry
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
114_UBI_iCre_crystGFP
Plasmid#171797PurposeTol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)DepositorInsertoptimised iCre under the control of the Ubiquitin promoter
PromoterUbiquitinAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
ExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-sgTrp53-sgAtrx-EFS-Cre
Plasmid#189977PurposeExpresses Cre cDNA and sgRNAs targeting murine Trp53 and AtrxDepositorInsertsgTrp53, sgAtrx, Cre recombinase
UseAAV, Cre/Lox, and Mouse TargetingAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ714
Plasmid#132955PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_A2S-8
Plasmid#188654PurposeAAV vector expressing SaAID-2S and gRNADepositorPublicationInsertsAID2S--nSaCas9-UGI
Sa-sgRNA
UseAAV and CRISPRTagsUGIExpressionMammalianMutationD10A for SaCas9PromoterScp1 and U6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-LN2
Plasmid#188235PurposePolycistronic human LIN28 and NANOG were cloned into the pMXs vectorDepositorAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
Tags6xHis tag and HRV3C cleavage siteExpressionBacterialPromoterT7Available sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-mCherry-NLS
Plasmid#186346PurposeNucleus-targeted mCherry expression in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-3xNLS
ExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIV489
Plasmid#177697PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by