We narrowed to 15,677 results for: Nes
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Plasmid#140102PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralPromotergRNA1 under U6 and gRNA2 under H1Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR10
Plasmid#167003PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDestTol2-actinb-kcnk9-IRES-EGFP
Plasmid#164949PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFPDepositorInsertkcnk9 (kcnk9 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164042PurposeCRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA geneDepositorInsertmCherry-CRISPR-TagV2
UseCRISPRExpressionMammalianAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7612 MSCV-P C-FlagHA 16E7 E10K
Plasmid#163303PurposeExpresses HPV16 E7 E10KDepositorInsertHPV16 E7 E10K
UseRetroviralTagsFlagHAMutationE10KPromoterMSCV LTRAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Plasmid#159801PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1180 EACMCV DNA-B 1.6mer
Plasmid#159137PurposeInfectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 2007 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGria3
Plasmid#124869PurposeMutagenesis of Gria3DepositorInsertGria3 (Gria3 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
MDIS2/MRH1 P1_pECIA14
Plasmid#114839PurposePrey vector MDIS2/MRH1 P1_pECIA14 should be used with bait vector MDIS2/MRH1 P1_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SERK2 P5_pECIA14
Plasmid#114768PurposePrey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE
Plasmid#122055PurposeLentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domainDepositorUseLentiviralExpressionMammalianMutationMyoD chimeric protein substituted with the NeuroD…Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-TNFRSF10B
Plasmid#117373PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR TNFRSF10B
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMurine PGK-1Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303 prp8-101
Plasmid#111307Purposeprp8-101DepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_110μ-pcDNA3
Plasmid#111929PurposeCyan color bright luminescent calcium indicator with low affinityDepositorInsertCeNL(Ca2+)_110μ
ExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_19μ-pcDNA3
Plasmid#111927PurposeCyan color bright luminescent calcium indicator with intermediate affinityDepositorInsertCeNL(Ca2+)_19μ
ExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHKU38.2-2.2
Plasmid#110670PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
ExpressionYeastAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only