We narrowed to 15,978 results for: grna

1. pLKO.1-puro-shFAM134B

   Plasmid

   #109013

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   shFAM134B (RETREG1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS597

   Plasmid

   #188884

   Purpose

   dpy-10 sgRNA expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
   

   Expression

   Worm

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ141A

   Plasmid

   #69290

   Purpose

   Gateway entry vector; single gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG3

   Plasmid

   #162604

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   ermE*/tipA

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT30

   Plasmid

   #204294

   Purpose

   For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Promoter

   MHC

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRT2B-AdGO2

   Plasmid

   #136911

   Purpose

   Lentivirus expressing AdGO2 sgRNA

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   sgAdGO2
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   hU6

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSWAP_Entry_T2

    Plasmid

    #184864

    Purpose

    pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSWAP_Entry_T1

    Plasmid

    #184863

    Purpose

    pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCR-Zeo

    Plasmid

    #158709

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Expression

    Bacterial

    Promoter

    Hsp60

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-Lb-crRNA

    Plasmid

    #78957

    Purpose

    Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB07

    Plasmid

    #190481

    Purpose

    Targeting plasmid for 2-plasmid C. difficile mutagenesis system

    Depositor

    Joseph Sorg

    Article

    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

    Inserts

    Upstream & Downstream pyrE deletion region
    gRNA
    

    Use

    E. coli / b. subtilis - c. difficile shuttle vect…

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6_crRNA_mCherry

    Plasmid

    #197609

    Purpose

    Expresses a Cas7-11 crRNA and mCherry

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    U6_crRNA_mCherry
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. JME4599

    Plasmid

    #129660

    Purpose

    NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ462

    Plasmid

    #187970

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-petRNA

    Plasmid

    #181802

    Purpose

    petRNA cloning plasmid

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    petRNA-Kan
    

    Use

    CRISPR

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GESTALT_pX330-v1

    Plasmid

    #103061

    Purpose

    plasmid px330 with guide targeting GESTALT v1 through V5 constructs

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
    

    Use

    Lentiviral

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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