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pAAV_BiCHATe27_C1V1_eYFP

Plasmid

#213831

Purpose

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

C1V1-eYFP

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_jGCaMP8m

Plasmid

#213918

Purpose

AAV construct expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8m

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1

Insert

jGCaMP8m

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_Gq_dTomato

Plasmid

#213938

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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TR-GFP

Plasmid

#80591

Purpose

Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Publication

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

CBA

Available since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Publication

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a(+)-Histag-Cerulean (526)

Plasmid

#70716

Purpose

Expression vector for Cerulean purification

Depositor

Richard Murray

Publication

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pXP722

Plasmid

#46056

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

GAL1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pC210

Plasmid

#65950

Purpose

Weak Repressor plasmid for two-strain system

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

CinI

Tags

LAA tagged

Expression

Bacterial

Promoter

Prhl/lac-w

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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TLD_062

Plasmid

#19698

Depositor

James Collins

Publication

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pSIN4-LSM14A

Plasmid

#240022

Purpose

Expresses human LSM14A fused to 3X Flag in mammalian cells

Depositor

Rui Su

Publication

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

LSM14A

Use

Lentiviral

Tags

3X Flag

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_GCaMP6f

Plasmid

#213859

Purpose

AAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

GCaMP6f

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_dTomato_nlsdTomato

Plasmid

#218792

Purpose

AAV construct with dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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SL-SunTag

Plasmid

#196919

Purpose

The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

Depositor

Jeffrey Chao

Publication

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Publication

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Tags

MGH

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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SHC003BSD

Plasmid

#133300

Purpose

The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

Depositor

Jingshi Shen

Publication

Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-HH1713

Plasmid

#124994

Purpose

E. coli expression of recombinant H. hepaticus antigen HH_1713

Depositor

Dan Littman

Publication

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH_1713

Expression

Bacterial

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-22

Plasmid

#120001

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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