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PDGFRB gRNA (BRDN0001147116)

Plasmid

#77060

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRB (PDGFRB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PDGFRB gRNA (BRDN0001146738)

Plasmid

#77062

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDGFRB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDGFRB (PDGFRB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCB gRNA (BRDN0001148507)

Plasmid

#75954

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCB (PRKCB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCB gRNA (BRDN0001144990)

Plasmid

#75955

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCB (PRKCB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK2 gRNA (BRDN0001148477)

Plasmid

#76911

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK2 (RIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK4 gRNA (BRDN0001147121)

Plasmid

#75969

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK4 (RIPK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCA gRNA (BRDN0001148197)

Plasmid

#75924

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCA (PRKCA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCA gRNA (BRDN0001149352)

Plasmid

#75925

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCA (PRKCA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pfast bac cry1myc

Plasmid

#51892

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

cry1 (Cry1 Mouse)

Tags

3x Flag and myc

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
PDPK1 gRNA (BRDN0001162206)

Plasmid

#77451

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDPK1 (PDPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PDPK1 gRNA (BRDN0001162582)

Plasmid

#77450

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDPK1 (PDPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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NME1 gRNA (BRDN0001145504)

Plasmid

#75537

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MPP1 gRNA (BRDN0001148092)

Plasmid

#77805

Purpose

3rd generation lentiviral gRNA plasmid targeting human MPP1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MPP1 (MPP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-CENPB-N-22

Plasmid

#56155

Purpose

Localization: Nucleus/Centromeres, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

NM_001810.5 (CENPB Human)

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PBK gRNA (BRDN0001145450)

Plasmid

#75572

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001147308)

Plasmid

#75574

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TJP2 gRNA (BRDN0001147569)

Plasmid

#77827

Purpose

3rd generation lentiviral gRNA plasmid targeting human TJP2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TJP2 (TJP2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TJP2 gRNA (BRDN0001148019)

Plasmid

#77828

Purpose

3rd generation lentiviral gRNA plasmid targeting human TJP2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TJP2 (TJP2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge