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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X10

Plasmid

#226552

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X10

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X10

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr-mNeon

Plasmid

#194534

Purpose

Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42K-TDH3pr-mNeon

Plasmid

#194535

Purpose

High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N-TDH3pr-mNeon

Plasmid

#194536

Purpose

Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42N-TDH3pr-mNeon

Plasmid

#194537

Purpose

High copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41H-TDH3pr-mNeon

Plasmid

#194538

Purpose

Low copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-L1374

Plasmid

#226275

Purpose

Plasmid expressing the SEC18 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-S288C

Plasmid

#226274

Purpose

Plasmid expressing the SEC18 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-NCYC110

Plasmid

#226277

Purpose

Plasmid expressing the SEC18 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-UWOPS872421

Plasmid

#226276

Purpose

Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-S288C

Plasmid

#226266

Purpose

Plasmid expressing the SCT1 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-L1374

Plasmid

#226267

Purpose

Plasmid expressing the SCT1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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