We narrowed to 5,868 results for: nls

1. pT2-7xTcf-NLS-ONL-CP

   Plasmid

   #65716

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2-7xTcf-NLS-YNL-CP

   Plasmid

   #65714

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. RPL25NLS-GFP-PRA (102)

   Plasmid

   #24041

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Use

   Tags

   EGFP and PRA

   Expression

   Yeast

   Mutation

   Only bp 1-135 (aa 1-45) of RPL25

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pT2-7xTcf-NLS-CNL-CP

   Plasmid

   #65715

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol1-uas-mAKT-2a-nlstdTomato

   Plasmid

   #67706

   Purpose

   Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   mAKT-2A-nlstdTomato
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

   Plasmid

   #172195

   Purpose

   AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   transcription regulatory domain removed

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pattB-nsyb-MKIIK11A::nlsLexADBDo

   Plasmid

   #64726

   Purpose

   generating transgenic TRIC flies

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   MKIIK11A::nlsLexADBDo
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptagBFP-TetR-NLS-VP16

   Plasmid

   #103835

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-TetR-NLS-VP16 (tetR )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-ABEMAX-NLS-P2A_BLAST

   Plasmid

   #135355

   Purpose

   Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-2XNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid

    #89743

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi dark-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid

    #89742

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi lit-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mPA-GFP-PCNA-19-SV40NLS-4

    Plasmid

    #57154

    Purpose

    Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pAAV-S5E8-dTom-nlsdTom

    Plasmid

    #135643

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid

    #85821

    Purpose

    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    Zf Ubi::cMyc oDam_f_GFPoNLS
    

    Use

    Tol2 based vector

    Tags

    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

    Expression

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-FUS ΔNLS 1-513

    Plasmid

    #44984

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS ΔNLS (1-513aa) (FUS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 1-513

    Promoter

    tac

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pT2-7xTcf-NLS-YNL

    Plasmid

    #65711

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL
    

    Use

    Luciferase; Tol2 system

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGGEV_2_+nls-eGFP-HA-_+1_BK+

    Plasmid

    #49312

    Purpose

    Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

    Depositor

    Joachim Wittbrodt

    Article

    Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

    Insert

    nls-EGFP-HA
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

    Plasmid

    #67687

    Purpose

    UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Use

    Tags

    6xmyc-NLS-tdTomato-2A

    Expression

    Mutation

    S159A, S196A

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PHO4NLS-GFP-PRA (109)

    Plasmid

    #24044

    Depositor

    Michael Rout

    Insert

    PHO4
    

    Use

    Tags

    EGFP and PrA

    Expression

    Yeast

    Mutation

    Only bp 421-588 of PHO4 (NLS)

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pPHR-EYFP-NLS-VP16

    Plasmid

    #103822

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-VP16 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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