We narrowed to 10,911 results for: NSI

1. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. myr-palm_mCherry2-mEGFP

   Plasmid

   #127816

   Purpose

   Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   myristoylated-palmitoylated-mCherry2-mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-N1-ro1GFP

   Plasmid

   #82369

   Purpose

   Expression of redox sensitive GFP in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S/F64L/T65S/S147C/Q204C

   Promoter

   CMV

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS267

   Plasmid

   #122380

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET26b_Nb.AT110.i1

   Plasmid

   #135481

   Purpose

   E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110.i1
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRF0G-Tyr

   Plasmid

   #113644

   Purpose

   Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   MjYRS and tRNA gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. myr-palm_2xmEYFP

   Plasmid

   #127811

   Purpose

   Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   myristoylated-palmitoylated-mEYFP-mEYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-ro2GFP

   Plasmid

   #82370

   Purpose

   Expression of redox sensitive GFP in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro2GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C48S/F64L/S147C/Q204C

   Promoter

   CMV

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-Chimeric_Butterfly_CY_1.0

    Plasmid

    #59800

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Thy1.1-muSOX

    Plasmid

    #131702

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSETB-ro2GFP

    Plasmid

    #82366

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/S65T/Q80R/S147C/Q204C

    Promoter

    T7

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUE1-TSP-UL32

    Plasmid

    #162658

    Purpose

    Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-PA(WT)-Vav2

    Plasmid

    #86977

    Purpose

    PI(DM): Photo-activatable/light sensitive

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pADtet-His6-MscL

    Plasmid

    #83373

    Purpose

    Expresses wild type MscL in mammalian cells

    Depositor

    Allen Liu

    Article

    Heureaux et al Cell Mol Bioeng. 2014 Sep;7(3):307-319.

    Insert

    Mechanosensitive channel of large conductance (mscL E. coli)
    

    Use

    Adenoviral

    Tags

    His6 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. myr-palm_mCherry-mEGFP

    Plasmid

    #127815

    Purpose

    Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-muSOX

    Plasmid

    #131701

    Purpose

    expresses muSOX with GFP N-terminally fused

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    GFP-muSOX (GAMMAHV.ORF37 MHV68)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-iRFP

    Plasmid

    #64887

    Purpose

    Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

    Depositor

    Tomomi Ichinose

    Article

    Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

    Insert

    iRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. K8.1 Pr pGL4.16

    Plasmid

    #120377

    Purpose

    Firefly luciferase reporter with KSHV K8.1 late gene promoter

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    K8.1 Promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    K8.1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Chimeric_Butterfly_YR_1.0

    Plasmid

    #59801

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge