We narrowed to 5,837 results for: dna plasmids

1. DNA-CARtcr-IRESpuro

   Plasmid

   #89346

   Purpose

   2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
   

   Use

   Lentiviral

   Tags

   SNAPf tag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HCMVgH (Merlin)

   Plasmid

   #136448

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.0_LynSNAP IRES EGFP

   Plasmid

   #136625

   Purpose

   Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   Lyn11-SNAP-Tag (IRES EGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Gα13(EE)-YFP

   Plasmid

   #171374

   Purpose

   Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Ga13(EE)-YFP
   

   Tags

   Glu-Glu

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1_SM-FLuc0-malat1

   Plasmid

   #210580

   Purpose

   Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_SM-FLuc100-malat1

   Plasmid

   #210581

   Purpose

   Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
   

   Use

   Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_ spike_del19

   Plasmid

   #155297

   Purpose

   Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

   Depositor

   Raffaele De Francesco

   Article

   Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

   Insert

   SARS-Cov2 spike_deleted (S SARS-Cov2)
   

   Use

   Generation of sars-cov-2-pseudotyped lentiviral p…

   Mutation

   Deletion in C-term 19 aa of spike

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-tvD-Cit-tevs-DHFR

   Plasmid

   #116054

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   tvD-Cit-tevs-DHFR
   

   Expression

   Mammalian

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

   Plasmid

   #222861

   Purpose

   This plasmid codes for the OgeuIscB nickase

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB Nickase
   

   Use

   CRISPR

   Tags

   3X HA and Nucleoplasmin NLS and ITR2

   Expression

   Mammalian

   Mutation

   E193A for nickase mutation

   Promoter

   CMV

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CarO-BFP2-TSERex

    Plasmid

    #124795

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    CarO-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Mac-BFP2-TSERex

    Plasmid

    #124793

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Mac-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. DNA-PKcs N-terminal sgRNA

    Plasmid

    #207093

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGCGGGACTCGGCGGCATGG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT/TO MycLAP-STIL

    Plasmid

    #80266

    Purpose

    mammalian expression plasmid for MycLAP-STIL

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    Myc-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Tras light chain

    Plasmid

    #216294

    Purpose

    Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Char-BFP2-TSERex

    Plasmid

    #124792

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    Char-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-39S Light chain

    Plasmid

    #216297

    Purpose

    Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    39S Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MF3958 Light chain

    Plasmid

    #216304

    Purpose

    Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MF3958 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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