We narrowed to 39,881 results for: lat

1. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B Human, Synthetic, HDR template)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIZ GFP PODXL 5N>Q

   Plasmid

   #202419

   Purpose

   Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   N33/43/104/144/360Q

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-BLAST flag-CAD S1859A

   Plasmid

   #169821

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859A; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-ZIP(RK)

   Plasmid

   #27136

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Arginines in the SH2 domains mutated to lysines (…

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

   Plasmid

   #75081

   Purpose

   An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

   Insert

   Mem-AcGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Palmitylation site

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-ZIP(FF)

   Plasmid

   #27135

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Tyrosines in ITAMS 1-3 mutated to phenylalanines …

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIR-Report-RASA1-3'UTR

   Plasmid

   #62575

   Purpose

   Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   RASA1 (RASA1 Human)
   

   Use

   Luciferase

   Tags

   None

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMXS-IRES-BLAST flag-CAD S1406A

   Plasmid

   #169820

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1406A; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXS-IRES-BLAST flag-CAD S1859E

    Plasmid

    #169822

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859E; TCCC -> AGTC silent mutations at nt527…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-2T-CBP(1603-2678)

    Plasmid

    #183774

    Purpose

    Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    nej (nej Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

    Plasmid

    #169826

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A S1859A; TCCC -> AGTC silent mutat…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-BLAST flag-CAD T456A S1406A

    Plasmid

    #169823

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXS-IRES-BLAST flag-CAD T456A S1859A

    Plasmid

    #169824

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1859A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-AAV9-X1.1

    Plasmid

    #196836

    Purpose

    non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV9-X1.1 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available Since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLEX307_CR-PDHA2S293A

    Plasmid

    #115204

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX307_CR-PDHA2S291A/S293A

    Plasmid

    #115205

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    c.321C>T (silent when translated to protein, c…

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid

    #115187

    Purpose

    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    

    Use

    Lentiviral

    Tags

    Versatile affinity tag (2XStreptactin II-6XHis-3X…

    Mutation

    c.150G>A (silent when translated to protein, c…

    Promoter

    CMV

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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