We narrowed to 20,115 results for: IRE;

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pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

Plasmid

#191647

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT54

Plasmid

#218726

Purpose

Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

Insert

IFT54 (TRAF3IP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pVenus-Sec16A

Plasmid

#36157

Purpose

Expresses Sec16A as a Venus FP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

Venus (EYFP derivative)

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-EQ-mycStrep

Plasmid

#31838

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

E578Q

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-2T FRB S2035I

Plasmid

#26608

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

GST

Expression

Bacterial

Mutation

FRB domain only (aa 2015-2114); S2035I

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6P1-sr1(SIRPT1)

Plasmid

#75297

Purpose

Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 84-339

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_CD19.4

Plasmid

#140101

Purpose

CRISPR-Cas9 Knock down

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase C2029A

Plasmid

#59822

Purpose

Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2029A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + FlaAAA

Plasmid

#84867

Purpose

Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
FlaAAA

Use

Baculovirus

Tags

6xHis

Expression

Insect

Mutation

3 C-terminal leucines have been replaced by alani…

Promoter

Polyhedrin

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-AMPK-β2

Plasmid

#40604

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
lenti-GBA-N370S-tmpknot-epegRNA

Plasmid

#214091

Purpose

Lentiviral vector expressing epegRNA to induce GBA N370S mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

GBA N370S epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGK401red-hENT1

Plasmid

#166028

Purpose

Expresses human equilibrative nucleoside transporter 1 (hENT1)

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

human equilibrative nucleoside transporter 1 (SLC29A1 Human)

Use

Synthetic Biology

Expression

Yeast

Promoter

pPGK1

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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5K-tails-->R GLUT1-GFP

Plasmid

#200099

Purpose

human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 451, 456,…

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-PAZ(aa1-480)

Plasmid

#21529

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa1-480

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pCK389.AAV

Plasmid

#177327

Purpose

aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Inserts

SoxS
dCas9

Use

CRISPR

Tags

MCP (MS2 Coat Protein)

Mutation

R93A,S101A

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

Plasmid

#67292

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Myt1L (Myt1l Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-SRF

Plasmid

#98618

Purpose

Gateway entry TOPO TA cloning vector for SRF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Topo ta cloning vector

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

Plasmid

#191635

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_POU1F1_P2A_Hygro_Barcode

Plasmid

#120473

Purpose

Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU1F1 (POU1F1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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