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pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSIBR005

Plasmid

#177668

Purpose

Constitutive expression of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSIBR001

Plasmid

#177628

Purpose

SIBR Intron 1 (Int1) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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RhoU

Plasmid

#55698

Purpose

expression of mouse RhoU

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoU (Rhou Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pAV0756

Plasmid

#133508

Purpose

Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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997 pSG5L HA PTEN T382A

Plasmid

#10757

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

T382A

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pWZL blast goosecoid

Plasmid

#14095

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0764

Plasmid

#133520

Purpose

Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 4

Plasmid

#59977

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only
Psicheck2 TNC full length 3'UTR

Plasmid

#26995

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

TNC Full length 3' UTR (TNC Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
PMV-ycrtYB

Plasmid

#65343

Purpose

systhesis of crtYB gene in the beta-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtYB

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TF101-STTM-miR156/157

Plasmid

#84811

Purpose

Turning off/down miR156/157 in Soybean

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM-miR156/157

Expression

Plant

Promoter

unknown

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DKAR-Nuc

Plasmid

#45499

Depositor

Alexandra Newton

Article

Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

Insert

DKAR-Nuc

Tags

CFP, NLS, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
AAV_A2S-8

Plasmid

#188654

Purpose

AAV vector expressing SaAID-2S and gRNA

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S--nSaCas9-UGI
Sa-sgRNA

Use

AAV and CRISPR

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

Scp1 and U6

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-CD36-C-10

Plasmid

#57357

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD36 (CD36 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Tags

GST and thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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998 pSG5L HA PTEN T383A

Plasmid

#10758

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

T383A

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
mOrange2-Vinculin-23

Plasmid

#57978

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vinculin (VCL Human)

Tags

mOrange2

Expression

Mammalian

Mutation

V234L in vinculin

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only