We narrowed to 10,989 results for: T7

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pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDB267

Plasmid

#80695

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHHH_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHHH_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB278

Plasmid

#80694

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHH_44

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHH_44

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB294

Plasmid

#80693

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHEEE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHEEE_02

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB199

Plasmid

#80691

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB289

Plasmid

#80690

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEHE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEHE_02

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB185

Plasmid

#80688

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEH_04

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB285

Plasmid

#80687

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEEH_04

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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MK08_HUMAN_D0

Plasmid

#79695

Purpose

This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK08 (MAPK8 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK11_HUMAN_D0

Plasmid

#79692

Purpose

This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK11 (MAPK11 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P27A)

Plasmid

#62068

Purpose

Expression of human p53 (1-73) (P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P27A

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pRBC-PINBodyN-Tau(1-441)-404TAG

Plasmid

#253347

Purpose

Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 1-441, TAG codon at residue 404 for phosphoserine encoding

Depositor

Richard Cooley

Article

Vesely et al Protein Sci. 2026 Apr;35(4):e70520. doi:

Insert

Tau 2N4R S404 TAG (MAPT Human)

Tags

PINBodyN (his6-tag included)

Expression

Bacterial

Mutation

Serine 404 to TAG codon

Promoter

T7

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pRBC-PINBodyC-Tau(297-441)-WT-his6

Plasmid

#253344

Purpose

Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 297-441

Depositor

Richard Cooley

Article

Vesely et al Protein Sci. 2026 Apr;35(4):e70520. doi:

Insert

Tau 2N4R (MAPT Human)

Tags

His6 and PINBodyC

Expression

Bacterial

Mutation

deleted amino acids 1-296

Promoter

T7

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-MeCP2-R168X (pc4734 )

Plasmid

#249081

Purpose

Bacteria expression plasmid of MeCP2-R168X (aa1-aa167)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Expression

Bacterial

Mutation

MeCP2-R168X (aa1-aa167)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-MeCP2-R255X (pc4735)

Plasmid

#249082

Purpose

Bacteria expression plasmid of MeCP2-R255X (aa1-aa254)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Expression

Bacterial

Mutation

MeCP2-R255X (aa1-aa254)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2-R168X (pc4742 )

Plasmid

#249084

Purpose

Bacteria expression plasmid of GFP tagged MeCP2-R168X (aa1-aa167)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Mutation

MeCP2-R168X (aa1-aa167)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTYB1-GFP-MeCP2-R255X (pc4743 )

Plasmid

#249085

Purpose

Bacteria expression plasmid of GFP tagged MeCP2-R255X (aa1-aa254)

Depositor

Cristina Cardoso

Article

Zhang et al Nucleus. 2022 Dec;13(1):1-34. doi: 10.10

Insert

MeCP2 (MECP2 Human)

Tags

GFP

Expression

Bacterial

Mutation

MeCP2-R255X (aa1-aa254)

Promoter

T7

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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