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pOX-twk43

Plasmid

#16128

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (F32H5.2, twk-43 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCF226

Plasmid

#121667

Purpose

EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

cas9

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSL6 SMR1747

Plasmid

#28029

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutS (mutS E. coli)

Expression

Bacterial

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 34C

Plasmid

#17522

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-33

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Hy_pMtnA nLuc SV40

Plasmid

#132654

Purpose

Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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p3994 pGEM HHR23A

Plasmid

#10863

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

HHR23A (RAD23A Human)

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGGD007

Plasmid

#48837

Purpose

Provides linker:nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

linker:nuclear localization signal

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Pou2f3

Plasmid

#84321

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pou2f3 (Pou2f3 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRL1058

Plasmid

#70686

Purpose

Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1058

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pSL1315

Plasmid

#129527

Purpose

pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EPHA2 (3MBW)

Plasmid

#25357

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

epha2.023.326 (EPHA2 Human)

Tags

His

Expression

Insect

Available Since

Aug. 19, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pMGC

Plasmid

#112812

Purpose

PCR template vector for amplifying gRNA(F+E)-OstRNA fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

gRNAcore(F+E)-tRNA

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-4 porcupine (genomic DNA)

Plasmid

#37376

Depositor

Norbert Perrimon

Article

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Expression

Insect

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A[T22N]

Plasmid

#24906

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
-226bp goosecoid luciferase

Plasmid

#17158

Depositor

Ken Cho

Article

Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Insert

goosecoid promoter (gsc-a Frog)

Use

Luciferase

Tags

luciferase

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pISH-Emx1

Plasmid

#84309

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Emx1 (Emx1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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