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pENTR-TARDBP-G287A

Plasmid

#141323

Purpose

Gateway cloning of TARDBP-G287A

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-G287A (TARDBP Human)

Use

Gateway Cloning

Mutation

G287A

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

Plasmid

#162878

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K197R-VA

Plasmid

#191223

Purpose

Lentiviral expression of human IFIT5_K197R in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 197 to Arginine (K197R)

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_WT-VA

Plasmid

#191221

Purpose

Lentiviral expression of human IFIT5_WT in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K160R-VA

Plasmid

#191222

Purpose

Lentiviral expression of human IFIT5_K160R in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 160 to Arginine (K160R)

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc msfGFPΔC10-Utr28-222

Plasmid

#231558

Purpose

Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Utrophin calponin homology domain (UTRN Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)

Plasmid

#194175

Purpose

Expresses lncRNA HIF1a-AS1 mutant

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

HIF1a-AS1 (HIF1A-AS1 Human)

Expression

Mammalian

Mutation

Within Exon1, nucleotides 26-78 were removed

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta55-118-10xHis

Plasmid

#194180

Purpose

Expresses MPP8-delta55-118_10xHis

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta55-118 (chromo domain deletion)

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry- YAP-N-IDR

Plasmid

#166446

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, YAP-N-IDR(1-164) (YAP1, CRY2 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A1

Plasmid

#189749

Purpose

The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Plasmid

#207863

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Plasmid

#207860

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSW031

Plasmid

#231144

Purpose

Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

Depositor

Philip Cole

Article

Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

Insert

S. aureus Sortase A mutant

Tags

Nostoc punctiform split intein C-terminal fragmen…

Expression

Bacterial

Mutation

P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

Promoter

T7

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Tags

His-tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc FL

Plasmid

#221853

Purpose

Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAGE2.0

Plasmid

#165984

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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