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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJR2955

Plasmid

#122550

Purpose

Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemNeptune2.5

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJR2956

Plasmid

#122533

Purpose

Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV100

Plasmid

#110718

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJ23101

Plasmid

#70252

Purpose

Expresses YFP from J23101 promoter and CFP from standard J23101 promoter

Depositor

Jim Haseloff

Article

Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

Insert

promoter J23101-RBS B0034-EYFP-B0015

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLas81-LuxI

Plasmid

#73445

Purpose

HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL

Depositor

Jim Haseloff

Article

Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

Insert

LuxI

Use

Synthetic Biology

Promoter

pLas81

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Cerulean (526)

Plasmid

#70716

Purpose

Expression vector for Cerulean purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pXR003_puroR

Plasmid

#219819

Purpose

hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

Depositor

Philipp Maass

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.1

Plasmid

#218822

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rScarlet

Plasmid

#205759

Purpose

Expression of rScarlet in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rScarlet

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ11444

Plasmid

#183628

Purpose

pHR-R(AP1-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_774

Plasmid

#176659

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-GM-CSF

Plasmid

#174596

Purpose

expresses secreted murine GM-CSF

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

GM-CSF

Expression

Mammalian

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Consensus

Plasmid

#176664

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Agam_695

Plasmid

#176654

Purpose

Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MTK3_008

Plasmid

#123723

Purpose

Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS-VP16-EL222

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mCerulean3

Plasmid

#127592

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Zebrafish expression

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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