We narrowed to 61,621 results for: promoter
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Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-sACE2v2.4(732)-IgG1
Plasmid#154106PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease and neck domains …PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP - FLAG-APEX2- hs Lamin B1
Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 (LMNB1 Human)
Tags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-iFlpV
Plasmid#140137Purposecan be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinaseDepositorHas serviceAAV1 and AAV PHP.eBInsertiFlpV
UseAAVPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK12_IDG-K
Plasmid#135281PurposeGateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK12 (CDK12 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
Plasmid#134859PurposeExpresses EGFP-tagged ERGIC markerDepositorAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmiRFP680-tubulin
Plasmid#136575PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP680DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP680-tubulin
TagsmiRFP680ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
EB3-3xmNeonGreen-N1
Plasmid#129605PurposeIn vivo visualization of microtuble plus ends (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB3
ExpressionMammalianPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Puro
Plasmid#111886PurposeLentiviral expression of cDNA with puromycin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RET-WT
Plasmid#125252PurposeExpresses human RET in mammalian cellsDepositorAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SYP-HRP
Plasmid#117185PurposePlasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAVExpressionMammalianPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GACh2.0
Plasmid#121921Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0DepositorInsertGPCR activation based ACh sensor GACh2.0
UseAAVPromoterhSynAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneTagsFLAG and GSTExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109
Plasmid#111730PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ109 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBbsr-JNK KTR-mCherry
Plasmid#115493PurposeExpression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJNK KTR-mCherry
TagsmCherryExpressionMammalianMutationmCherry G229L, mCherry Y298 deletedPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBTet_NAD-Snifit-cyto
Plasmid#113914PurposePlasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNAD-Snifit
ExpressionMammalianPromoterCMV TetO2Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B-LIRmut
Plasmid#109027Purposestable lentiviral expression of cDNADepositorInsertFAM134B-LIRmut (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDDFELL to DDAEAL aa 453-458 to interrupt FAM134B …PromoterEIF-1a promoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only