We narrowed to 19,999 results for: IRE

Showing: 10281 - 10300 of 19999 results

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pcDNA3-HA-CUL1 L756A/I757A

Plasmid

#19938

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 L756A/I757A (CUL1 Human)

Tags

HA2

Expression

Mammalian

Mutation

Mutation of two adjacent hydrophobic residues, L…

Available Since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

Plasmid

#146553

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9GG

Plasmid

#131009

Purpose

Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of SpCas9
promoter PJ23119
mRFP expression cassette

Use

CRISPR

Expression

Bacterial

Promoter

Bba_R0040 TetR

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pY036_ATP1A1_G5

Plasmid

#86618

Purpose

Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G5 crRNA + AsCpf1-3xHA

Use

CRISPR; Co-selection via hdr using ouabain

Tags

3xHA

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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XE157 GFP-XDpr-delta PDZB-CS2+

Plasmid

#16765

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

gfp

Mutation

XDpr is truncated to delete the last four amino a…

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dicer_2

Plasmid

#68808

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PCD FL1P

Plasmid

#12567

Depositor

Carl Novina

Article

Wang et al Mol Cell. 2006 May 19. 22(4):553-60.

Insert

One perfect CXCR4 binding site (CXCR4 Human)

Use

Luciferase; Reporter for translation repression

Tags

luciferase

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pCRII-Sema4a-ISH-2kb

Plasmid

#68029

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4A (Sema4a Mouse)

Use

Mrna in situ hybridization probe

Mutation

a 2kb cDNA fragment for ISH probe synthesis

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCSP1-mp53AS

Plasmid

#20896

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

5' natural EcoRV and SacI restriction sites …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
L309-myc-seipin-CT

Plasmid

#64063

Purpose

myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

Insert

bscl2 (Bscl2 Mouse)

Use

Lentiviral

Tags

myc epitope

Expression

Mammalian

Mutation

Contains Arg326 to Ser443

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
(81) pcDNA3.1-BC2-Nup62-Flag-EPEA

Plasmid

#168094

Purpose

Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Tags

BC2-Tag, EPEA-tag, and Flag-tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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hs-lambda btl

Plasmid

#17581

Depositor

Denise Montell

Article

Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

Insert

lambda-btl (btl Fly)

Expression

Insect

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBluescript-mouse eIF2C1

Plasmid

#50353

Purpose

Mouse eIF2C1 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C1 (Eif2c1 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGEMT-Tyrosinase-bait-T2a-Cherry-pA

Plasmid

#111155

Purpose

knock in bait for Axolotl Tyrosinase locus

Depositor

Elly Tanaka

Article

Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

Insert

Tyrosinase-bait-T2a-Cherry-pA

Expression

Mammalian

Mutation

wt

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1 WT

Plasmid

#114049

Purpose

Expression of exogenous LacI-GFP-HEC1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

WT HEC1 sequence

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJS278

Plasmid

#134139

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255, R155A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pVMG0173_Cq-Actin5c-Cas9

Plasmid

#169345

Purpose

Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0173_Cq-Actin5c-Cas9

Use

CRISPR

Expression

Insect

Promoter

CPIJ009808

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/SH/GW-Mst1

Plasmid

#84299

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst1 (STK4 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only