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pWN080

Plasmid

#233820

Purpose

MoClo-YTK; type3B; NS3 systems; Linker-NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Linker-NS3a

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN062

Plasmid

#233822

Purpose

MoClo-YTK; type3A; NS3 systems; LexA-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

LexA-NES

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO176

Plasmid

#233824

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCDS.AEBP2.I3

Plasmid

#234573

Purpose

Coding sequence for AEBP2 Isoform 3

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

AEBP2 [Isoform 3] (AEBP2 Human)

Use

Unspecified

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCDS.SUZ12

Plasmid

#234572

Purpose

Coding sequence for SUZ12

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

SUZ12 (SUZ12 Human)

Use

Unspecified

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDS.EED

Plasmid

#234569

Purpose

Coding sequence for EED

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EED (EED Human)

Use

Unspecified

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDS.RBBP4

Plasmid

#234571

Purpose

Coding sequence for RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

RBBP4 (RBBP4 Human)

Use

Unspecified

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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NarP-GFP

Plasmid

#220161

Purpose

To track the natural NarP promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

NarP promoter only

Expression

Bacterial

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Promoter

3XP3

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRC065d

Plasmid

#225979

Purpose

dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Inserts

dRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS

Use

CRISPR

Promoter

J23107 and J23119

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pUdO3c

Plasmid

#210232

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC10_pPromALB_STARRseq

Plasmid

#220289

Purpose

STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::luxI

Plasmid

#189574

Purpose

Lux synthase (3OC6-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::traI

Plasmid

#189575

Purpose

Tra synthase (3OC8-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::lasI

Plasmid

#189576

Purpose

Las synthase (3OC12-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSU19mCherry

Plasmid

#225181

Purpose

Medium copy cloning vector with mCherry integrated in the multiple cloning site. There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM255

Plasmid

#226899

Purpose

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-Prescission-GSS-…

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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