We narrowed to 18,496 results for: Met

1. pMO746

   Plasmid

   #117480

   Purpose

   upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

   Depositor

   Judy Wall

   Article

   Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

   Insert

   uracil phosphoribosyltransferase
   

   Expression

   Bacterial

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. rTetR-NS3-VP64-p65_IRES_mCherry

   Plasmid

   #112628

   Purpose

   Encodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

   Depositor

   John Ngo

   Article

   Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

   Insert

   rTetR-NS3-VP64-p65
   

   Tags

   AU1 (N term on NS3), HA (C term on NS3), and T7 (…

   Expression

   Mammalian

   Mutation

   NS3 mutant T54A

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-GFP-2A-G(N2c)-WPRE

   Plasmid

   #194352

   Purpose

   Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

   Depositor

   Marco Tripodi

   Article

   Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

   Inserts

   Enhanced Green Fluorescent Protein (eGFP)
   Rabies CVS-N2c glycoprotein (G)
   

   Use

   Lentiviral

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEos2-Integrin-Alpha2-N-18

   Plasmid

   #57389

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Integrin-Alpha2 (ITGA2 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

   Plasmid

   #124705

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD (E488Q)_NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutant

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMito-miRFP718nano

   Plasmid

   #195754

   Purpose

   Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   Human cytochrome C oxidase subunit VIII-miRFP718nano
   

   Tags

   miRFP718nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK2-miR-34 WT

   Plasmid

   #78258

   Purpose

   psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   fully complimentary miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Expression

   Mammalian

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pALS099 (pGEX-2TP-cTevHis-DEST)

   Plasmid

   #139787

   Purpose

   Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

   Depositor

   Jeannie Lee, Arneet Saltzman

   Article

   Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

   Type

   Empty backbone

   Tags

   6xHis (cleavable by TEV protease) and GST (cleava…

   Expression

   Bacterial

   Available Since

   April 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK1179

    Plasmid

    #179296

    Purpose

    Expresses dCas9 fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-DmrA(x4)
    

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC71100

    Plasmid

    #154068

    Purpose

    Level 0 Golden Gate vector, pHvHSP17

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    pHvHSP17
    

    Use

    Standard cloning vector used by geneart for synth…

    Mutation

    All BsaI, Esp3I and BpiI restriction sites were r…

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-NPY-ClopHensor

    Plasmid

    #25939

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
    

    Tags

    NPY and Strep-II

    Expression

    Mammalian

    Mutation

    Neuropeptide Y localization signal

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDx_mRuby3-SYFP2

    Plasmid

    #189758

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mRuby3-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH2B-miRFP718nano

    Plasmid

    #195750

    Purpose

    Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    H2B-miRFP718nano
    

    Tags

    miRFP718nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRetroSuper-shSrc

    Plasmid

    #26982

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Src (SRC Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pirespuro2-CD86-mEos2

    Plasmid

    #98284

    Purpose

    mammalian expression of CD86-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CD86 (CD86 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDendra2-H3

    Plasmid

    #157814

    Purpose

    Fluorescent reporter for histone H3 tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H3 (H3c1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcBac1-haloTyrRS C6

    Plasmid

    #141174

    Purpose

    Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyr tRNA synthetase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    CMV

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

    Plasmid

    #124626

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR1 DD (E1008Q)_NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E1008Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1 SENP6

    Plasmid

    #14583

    Depositor

    Mary Dasso

    Article

    Mukhopadhyay et al J Cell Biol. 2006 Sep 25. 174(7):939-49.

    Insert

    SENP6 (SENP6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 28, 2007

    Availability

    Academic Institutions and Nonprofits only