We narrowed to 88,799 results for: Mal

1. pRS0045

   Plasmid

   #131124

   Purpose

   Mammalian Target-ACE expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1, TadA, and UGI

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-FOG1[N+C]

   Plasmid

   #100085

   Purpose

   dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   FOG1(aa 1-45) (ZFPM1 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-FOG1-dCas9-FOG1-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1-FHC-POLQ-K121M

   Plasmid

   #64877

   Purpose

   lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   K121M, a mutation in the conserved ATP-binding si…

   Promoter

   EF1alpha

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. DRH229: FCK-QuasAr1-mO2

   Plasmid

   #51629

   Purpose

   Fluorescent reporter for membrane voltage with improved brightness

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr1-mOrange
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

   Plasmid

   #160046

   Purpose

   For expressing the full paradoxical circuit

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Inserts

   osTIR1
   iaaH
   iCasp9
   BlastR
   

   Use

   Synthetic Biology

   Tags

   mAID, mCherry, and mGFPmut3

   Expression

   Mammalian

   Promoter

   no promoter, T2A is used and pEF1s

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG AKT3 E17K

    Plasmid

    #73050

    Purpose

    Human AKT3 E17K

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    AKT3 E17K (AKT3 Human)
    

    Expression

    Mammalian

    Mutation

    E17K

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-short_BrokenHeart (no BC)

    Plasmid

    #86950

    Purpose

    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    Brokenheart construct
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG5-P190

    Plasmid

    #31285

    Depositor

    Nora Heisterkamp

    Article

    Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

    Insert

    Bcr-abl fusion protein P190 (ABL1, BCR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. PiggyBac-FLAG-mProser1-IRES-Blast

    Plasmid

    #226172

    Purpose

    Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

    Depositor

    Kasper Rasmussen

    Article

    Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

    Insert

    Proline Serine Rich protein 1 (Proser1 Mouse)
    

    Tags

    2X FLAG

    Expression

    Mammalian

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-TRE-FH-TET3 short

    Plasmid

    #166916

    Purpose

    Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    TET3 short isoform
    

    Use

    Lentiviral

    Tags

    HA Tag

    Expression

    Mammalian

    Mutation

    Changed nucleotide 18 (G) to (A) to delete FseI r…

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV302

    Plasmid

    #119943

    Purpose

    Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICOZ-SB100X[EF1α-CD19 4-1BB]

    Plasmid

    #218328

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

    Plasmid

    #161577

    Purpose

    Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Expression

    Mammalian

    Promoter

    EF1a driving selection cassette

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBa-flag-BicD2 594-FKBP

    Plasmid

    #64206

    Purpose

    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Bicaudal D protein (Bicd2 Mouse)
    

    Tags

    FKBP and flag

    Expression

    Mammalian

    Mutation

    no start methionine, aa1-594

    Promoter

    chicken Beta Actin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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