We narrowed to 10,981 results for: cat.1

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-LOV*-H2B

Plasmid

#202204

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Histone H2B type 1-K

Tags

FLAG and LOV*

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

Plasmid

#126552

Purpose

Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

CD8a and GFP

Mutation

Delta gag/pol

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCsA

Plasmid

#136067

Purpose

Acceptor plasmid for even level position 1, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-PTEN-shRNA-3001

Plasmid

#25639

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PTEN-shRNA-3001 (PTEN Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

Plasmid

#126553

Purpose

Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

Thy1.2 and GFP

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKL002

Plasmid

#98921

Purpose

pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Inserts

PROPS
GCaMP6f

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Map3k1

Plasmid

#29581

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Map3k1 (MAP3K1 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively Active. Contains catalytic domain …

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQ398X-EGFP

Plasmid

#68849

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q398X, results in truncation of the protein into …

Promoter

CMV

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pTK162_mCherry-4.1G-hardened

Plasmid

#46361

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Modified for siRNA resistance to gcTccTcaTttCgaAc…

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

Plasmid

#91932

Purpose

Bacterial expression of maize SUMO1a and SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-macroHC

Plasmid

#82337

Purpose

Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

Depositor

Michael Hothorn

Article

Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

Insert

SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)

Tags

6xHis non-cleavable and macro domain of human his…

Expression

Bacterial

Mutation

Truncated protein, amino acids 1-178

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

Plasmid

#91934

Purpose

Bacterial expression of maize SUMO1a and SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-MaUPPS-A

Plasmid

#203174

Purpose

Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

uppS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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C514-E11: attB5r-RU5u-His6-nostop-attB1r

Plasmid

#162953

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

Plasmid

#162961

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H-H1

Plasmid

#163584

Purpose

express Dcp2 FL-H1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300 AAAA

Plasmid

#163579

Purpose

express Dcp2 300 AAAA at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domain (301-970); mutati…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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