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T7_MBP_TEV_ISCro4_6xHis_T7_term

Plasmid

#251502

Purpose

Bacterial expression vector for producing recombinant ISCro4 (E. coli codon optimised)

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge recombinase

Expression

Bacterial

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgRNA-BFP-Puro

Plasmid

#229014

Purpose

Perturb Seq lentiviral sgRNA vector

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHDR-USR-SpCas9

Plasmid

#208829

Purpose

HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

Depositor

Zhiying Zhang

Article

Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBW-507

Plasmid

#195469

Purpose

Lentiviral Integratable Constitutive CD19CAR

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV-CD19CAR-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND1-Right-G1397-C

Plasmid

#180769

Purpose

Expresses mitoZFD_ND1-Right-G1397-C(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-CAG-EGFP

Plasmid

#153186

Purpose

CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pOZC-PALB2

Plasmid

#133552

Purpose

PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

Depositor

David Livingston

Article

Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

Insert

PALB2 (PALB2 Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(0)_T2AGAL4

Plasmid

#125211

Purpose

Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18

Plasmid

#79933

Purpose

PH-4GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-PBX1a

Plasmid

#78149

Purpose

mammalian expression of PBX1a

Depositor

Francesco Blasi

Insert

PBX1 (PBX1 Human)

Expression

Mammalian

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pRB37

Plasmid

#72869

Purpose

template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

Strep - His8 - Strep -TEV tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–EgRNA

Plasmid

#62716

Purpose

Empty; no U6 gRNA cassette

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2

Plasmid

#51848

Purpose

promoter region (-550;0) of PIM2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only

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pRS305:3mCherry

Plasmid

#37552

Depositor

Wei-Lih Lee

Article

Markus et al Curr Biol. 2009 Feb 10;19(3):196-205. Ep

Insert

3mCherry

Expression

Bacterial

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

Plasmid

#219828

Purpose

Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

Depositor

Philipp Maass

Type

Empty backbone

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMIA10.53-Clover

Plasmid

#214450

Purpose

BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-GFP-WPRE

Plasmid

#211488

Purpose

expresses GFP under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GFP

Use

AAV

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_PUUV_GP

Plasmid

#207301

Purpose

Mammalian expression of Puumala virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

PUUV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_LACV_GP

Plasmid

#207286

Purpose

Mammalian expression of La Crosse virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LACV_GP (M La Crosse virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_HCV_E1E2

Plasmid

#207279

Purpose

Mammalian expression of Hepatitis C virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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