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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 49,092 results for: prot

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Showing: 10321 - 10340 of 49092 results

  2. pETSG

    Plasmid
    #159418
    Purpose
    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli
    Depositor
    Dianfan Li
    Article
    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:
    Insert
    thermostable GFP (TGP)
    Tags
    polyhistidine (poly-His)
    Expression
    Bacterial
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    159418-plasmid-map-sequence-id-321533

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  9. H2B-LSSmGFP

    Plasmid
    #186539
    Purpose
    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Tags
    Human Histone 2B (D26G and V119I mutations)
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available Since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186539-plasmid-map-sequence-id-368219

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  11. FKBP_LD 0_TEV

    Plasmid
    #58877
    Purpose
    MESA protease chain with Rapamycin FKBP ectodomain and no linker domain
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    58877-plasmid-map-sequence-id-138316

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  12. pGL3-LRF

    Plasmid
    #66936
    Purpose
    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).
    Depositor
    Alvaro Monteiro
    Article
    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV-40
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  18. pBA2675

    Plasmid
    #189997
    Purpose
    Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)
    Depositor
    Bungo Akiyoshi
    Article
    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    NLS
    Mutation
    N-terminal 600 bp of FBP75
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189997-plasmid-map-sequence-id-371586

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Showing: 10321 - 10340 of 49092 results