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pCRISPEY-Z3-Nat-ADE2

Plasmid

#232106

Purpose

Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

Z3

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-Z3-Hyg-ADE2

Plasmid

#232107

Purpose

Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

Z3

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-PLAP

Plasmid

#222308

Purpose

Synchronize the trafficking of PLAP from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0034 CMVTO-hIL6-rens-hIL6Ra_M4

Plasmid

#231217

Purpose

C-CageIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6-rens-hIL6Ra_M4 (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0200 CMVTO-hIL6Ra_M4-rens-hIL6

Plasmid

#231193

Purpose

CageIL-6 (N-term)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6Ra_M4-rens-hIL6 (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0165 CMVTO-mono-hIL10 (R5A11M)-rens

Plasmid

#231196

Purpose

CleaveIL-10

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10 (R5A11M)-rens (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Aquamarine-LC3B G120A

Plasmid

#228563

Purpose

Encodes a donor-only, Aquamarine-tagged version of the inactive LC3B biosensor under the control of the CMV promoter

Depositor

Giulia Bertolin

Article

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

Aquamarine

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

Plasmid

#203447

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) L373A

Plasmid

#221242

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

L373A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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p204-U6-Switch-ON

Plasmid

#217883

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A341; A1171)

Plasmid

#204197

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1a (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 341, 1171

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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NES-mAzGreen-Cyclin D1 (HP)

Plasmid

#215070

Purpose

Expresses NES-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NES-mAzGreen

Expression

Mammalian

Mutation

Cyclin D1(HP) denotes mutations M56A, V60A, and W…

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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NLS-mAzGreen-Cyclin D1 (HP)

Plasmid

#215072

Purpose

Expresses NLS-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

NLS-mAzGreen

Expression

Mammalian

Mutation

Cyclin D1(HP) denotes mutations M56A, V60A, and W…

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (L115R)

Plasmid

#205717

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5 (L115R)-HaloTag

Plasmid

#205711

Purpose

Mammalian expression of DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1-L24_sfGFP

Plasmid

#207865

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1_sfGFP

Plasmid

#207801

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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