We narrowed to 617 results for: spen

1. PX458-sgTSC2

   Plasmid

   #128098

   Purpose

   CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   sgRNA against human TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-noSV40-humanEC1.45

   Plasmid

   #173946

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

   Plasmid

   #107010

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-Actin-C-18

   Plasmid

   #54863

   Purpose

   Localization: Actin, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pOPINK-ALG13 (OTU, aa 216-353)

   Plasmid

   #61414

   Purpose

   Expresses human ALG13 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-215 and aa 354-1137.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

   Plasmid

   #113160

   Purpose

   A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-IP-mCherry-TOSI

   Plasmid

   #172496

   Purpose

   mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   PDCD4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   Mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   MLV LTR

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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