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pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry

Plasmid

#85430

Purpose

[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human IL-10 T2A PD-L1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28A

Plasmid

#51371

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST26-N-FLAG

Plasmid

#79549

Purpose

Destination vector containing an N-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits
pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64

Plasmid

#85423

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-5 synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64

Plasmid

#85422

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her_4D5-3 synNotch

Use

Lentiviral

Promoter

PGK

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Tags

None

Expression

Bacterial

Promoter

Inducible Tac promoter

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pYESir2HY-puro

Plasmid

#1770

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

H363Y, residue 363 changed from histidine to tyro…

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-Mst2

Plasmid

#19056

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Mammalian Ste20-like kinase 2 (STK3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00532

Plasmid

#39630

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3629 (Dll Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
hsp70:Arl13b-mKate2

Plasmid

#105964

Purpose

heat shock inducible transgenesis, primary cillium marker

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Arl13b (Arl13b Mouse)

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSRalpha-MSV-mouse Cdk4wt-Tk-neo

Plasmid

#115525

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

Insert

Mouse Cdk4 (Cdk4 Mouse)

Use

Retroviral

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only
pMXs-NKX2.2

Plasmid

#134670

Purpose

Retroviral expression of mouse Nkx2.2

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCPP5060

Plasmid

#128716

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

Insert

shcN-hopN1

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pME GFP-PCNA

Plasmid

#105977

Purpose

entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

PCNA (PCNA Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUC Ad VAI

Plasmid

#175518

Purpose

For mammalian expression of adenovirus VAI RNA

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

Ad VAI RNA

Use

Adenoviral

Promoter

lac promoter

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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CMV/N3IC-HA

Plasmid

#47618

Purpose

in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

Depositor

Urban Lendahl

Article

Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

Insert

Notch3 Intracellular domain (Notch3 Mouse)

Use

In vitro transcription and translation

Tags

HA

Expression

Mammalian

Mutation

codons 1664 (Met) to 2318 (Ala)

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pRcCMV Cep164 (Nigg CW325)

Plasmid

#41148

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only