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pME-NLS-tdMCP

Plasmid

#86245

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLStdMCP

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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ZP45 Prickle-1

Plasmid

#16897

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMZ035

Plasmid

#66972

Purpose

Expresses zebrafish Chk1 mutant

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Mutation

mutated S256E, S280E, T292E, and S301E

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMZ030

Plasmid

#66970

Purpose

Expresses wild type zebrafish Chk1 with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

wildtype zebrafish Chk1

Tags

GFP

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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XE247 Xenopus CK1 epsilon K to R CS2+

Plasmid

#16725

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

casein kinase 1, epsilon

Use

Xenopus expression

Mutation

K38R

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pDestTol2-QUAS:GFP

Plasmid

#184811

Purpose

Used to generate the Tg(QUAS:GFP)c578 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHMG20B.1.0-gDNA

Plasmid

#132449

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Kevin White

Insert

HMG20B (HMG20B Human)

Use

CRISPR

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T ZfHoxc10a

Plasmid

#27536

Depositor

Victoria Prince

Article

Prince et al Development. 1998 Feb . 125(3):407-20.

Insert

Hoxc10a (hoxc10a Zebrafish)

Expression

Bacterial

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194518

Purpose

ZSP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-fos:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194516

Purpose

fos promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#193851

Purpose

E1b promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194515

Purpose

P2 promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-mfap4-BFP

Plasmid

#232188

Purpose

macrophage-specific promoter driving BFP in a Tol2 backbone for expression in zebrafish

Depositor

Emily Rosowski

Article

Thrikawala et al J Immunol. 2024 Oct 1;213(7):971-987. do

Insert

BFP

Use

Zebrafish expression

Promoter

mfap4

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ EGFP-3NLS

Plasmid

#165400

Purpose

Expresses nuclear localized EGFP

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

EGFP

Expression

Mammalian

Promoter

SP6

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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M28 Nac>GFP

Plasmid

#17178

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter (mitfa Zebrafish)

Tags

GFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pAF018 p3E-2A-mCerulean-CAAX

Plasmid

#195975

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann

Article

Kirchgeorg et al Dev Dyn. 2018 Oct;247(10):1146-1159. doi

Insert

2A-mCerulean-CAAX (membrane)

Use

Other

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX

Plasmid

#184813

Purpose

Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pME-NLS-tdMCP-eGFP

Plasmid

#86243

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP-eGFP

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2

Plasmid

#209282

Purpose

Expresses FR-GECO1c and CoChR in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2

Use

Zebrafish expression

Promoter

zACNE20_myl7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT7tyrgRNA

Plasmid

#46761

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

tyr gRNA (tyr Zebrafish)

Use

CRISPR

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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XE244 Mouse Naked-1 CS2p+

Plasmid

#16728

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Mouse Naked-1 (Nkd1 Mouse)

Use

Xenopus expression

Tags

His and myc

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pME-NLStdMCP- tagRFP

Plasmid

#86244

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP-tagRFP

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBJ077_zACNE20_E1b_2xLyn_mCherry

Plasmid

#209281

Purpose

Expresses membrane-labelled mCherry in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

mCherry2

Use

Zebrafish expression

Promoter

zACNE20_E1b

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2z

Plasmid

#62214

Purpose

pCS2 with antifreeze protein terminator instead of sv40

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Mrna synthesis

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pT7goldRNA

Plasmid

#47930

Purpose

In vitro transcription of golden gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

golden gRNA (slc24a5 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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hsp70:mKate2-NWASP-CA

Plasmid

#105967

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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TCRalpha-L1

Plasmid

#11262

Depositor

Lisa Steiner

Article

Danilova et al Dev Comp Immunol. 2004 Jun . 28(7-8):755

Insert

TCR alpha (trac Zebrafish)

Expression

Bacterial

Available Since

March 15, 2006

Availability

Academic Institutions and Nonprofits only